Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | relBE/ParE-DnaT |
| Location | 16426..16948 | Replicon | plasmid unnamed2 |
| Accession | NZ_CP055043 | ||
| Organism | Shigella flexneri strain SWHEFF_59 | ||
Toxin (Protein)
| Gene name | relE | Uniprot ID | G3CAN5 |
| Locus tag | HUZ71_RS23070 | Protein ID | WP_000220560.1 |
| Coordinates | 16667..16948 (+) | Length | 94 a.a. |
Antitoxin (Protein)
| Gene name | relB | Uniprot ID | G3CAG1 |
| Locus tag | HUZ71_RS23065 | Protein ID | WP_000121743.1 |
| Coordinates | 16426..16677 (+) | Length | 84 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| HUZ71_RS23045 (HUZ71_22860) | 12410..12763 | - | 354 | WP_000876434.1 | DNA distortion polypeptide 3 | - |
| HUZ71_RS23050 (HUZ71_22870) | 12900..13346 | - | 447 | WP_001074386.1 | hypothetical protein | - |
| HUZ71_RS23055 (HUZ71_22875) | 13386..14222 | - | 837 | WP_001050931.1 | RepB family plasmid replication initiator protein | - |
| HUZ71_RS23060 (HUZ71_22880) | 15260..16039 | + | 780 | Protein_21 | transposase | - |
| HUZ71_RS23065 (HUZ71_22885) | 16426..16677 | + | 252 | WP_000121743.1 | plasmid stabilization protein | Antitoxin |
| HUZ71_RS23070 (HUZ71_22890) | 16667..16948 | + | 282 | WP_000220560.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| HUZ71_RS23075 (HUZ71_22895) | 17094..17417 | + | 324 | WP_001181903.1 | hypothetical protein | - |
| HUZ71_RS23080 (HUZ71_22900) | 17462..17707 | + | 246 | WP_000356546.1 | hypothetical protein | - |
| HUZ71_RS23085 (HUZ71_22905) | 17697..17912 | + | 216 | WP_001180116.1 | hypothetical protein | - |
| HUZ71_RS23090 (HUZ71_22910) | 18005..18334 | + | 330 | WP_000866648.1 | hypothetical protein | - |
| HUZ71_RS23095 (HUZ71_22915) | 18621..18770 | + | 150 | WP_000003880.1 | hypothetical protein | - |
| HUZ71_RS23100 (HUZ71_22920) | 18783..19055 | + | 273 | WP_000160399.1 | hypothetical protein | - |
| HUZ71_RS23105 (HUZ71_22925) | 19382..19927 | + | 546 | WP_038976855.1 | DNA distortion polypeptide 1 | - |
| HUZ71_RS23110 (HUZ71_22930) | 19930..21087 | + | 1158 | WP_000538023.1 | DNA distortion polypeptide 2 | - |
| HUZ71_RS23115 (HUZ71_22935) | 21448..21939 | + | 492 | WP_000872475.1 | transcription termination/antitermination NusG family protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | ant(3'')-Ia / sul3 / blaTEM-176 / aph(3')-Ia / floR | - | 1..47227 | 47227 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 94 a.a. Molecular weight: 11005.83 Da Isoelectric Point: 10.5938
>T161192 WP_000220560.1 NZ_CP055043:16667-16948 [Shigella flexneri]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADLR
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADLR
Download Length: 282 bp
>T161192 NZ_CP071086:874601-875011 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAAATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAAATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA
Antitoxin
Download Length: 84 a.a. Molecular weight: 9194.43 Da Isoelectric Point: 4.1683
>AT161192 WP_000121743.1 NZ_CP055043:16426-16677 [Shigella flexneri]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL
Download Length: 252 bp
>AT161192 NZ_CP071086:874354-874620 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | G3CAN5 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A3S5I301 |