Detailed information of TA system TA161191

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 77564..78207
• Replicon: plasmid unnamed1
• Accession: NZ_CP055042
• Organism: Shigella flexneri strain SWHEFF_59

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: B1LRW4
• Locus tag: HUZ71_RS22900
• Protein ID: WP_001044768.1
• Coordinates: 77564..77980 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D2WFK3
• Locus tag: HUZ71_RS22905
• Protein ID: WP_001261287.1
• Coordinates: 77977..78207 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ71_RS22885 (HUZ71_22695)	74063..74653	-	591	WP_000194575.1	hypothetical protein	-
HUZ71_RS22890 (HUZ71_22700)	74653..74910	-	258	WP_000343085.1	hypothetical protein	-
HUZ71_RS22895 (HUZ71_22705)	75264..77402	+	2139	WP_000350638.1	AAA family ATPase	-
HUZ71_RS22900 (HUZ71_22710)	77564..77980	-	417	WP_001044768.1	type II toxin-antitoxin system VapC family toxin	Toxin
HUZ71_RS22905 (HUZ71_22715)	77977..78207	-	231	WP_001261287.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
HUZ71_RS22910 (HUZ71_22720)	78503..78793	+	291	WP_000111771.1	hypothetical protein	-
HUZ71_RS22915 (HUZ71_22725)	78783..79682	+	900	WP_000963206.1	nucleotide-binding protein	-
HUZ71_RS22920 (HUZ71_22730)	79732..81264	-	1533	WP_239638078.1	P-loop NTPase fold protein	-
HUZ71_RS22925 (HUZ71_22735)	81419..81607	+	189	WP_000957857.1	hypothetical protein	-
HUZ71_RS22930 (HUZ71_22740)	81617..82816	+	1200	WP_000948429.1	IS91 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	sul2 / aph(3'')-Ib / aph(6)-Id / tet(A) / blaTEM-176 / mph(A) / sul1 / qacE / aadA2 / dfrA12 / tet(M)	-	1..87472	87472
	-	flank	IS/Tn	-	-	81617..82816	1199

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15197.66 Da
• Isoelectric Point: 7.7805

>T161191 WP_001044768.1 NZ_CP055042:c77980-77564 [Shigella flexneri]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Nucleotide

• Length: 417 bp

>T161191 NZ_CP071086:741538-742023 [Klebsiella pneumoniae]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGCCCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCCCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8815.90 Da
• Isoelectric Point: 4.3775

>AT161191 WP_001261287.1 NZ_CP055042:c78207-77977 [Shigella flexneri]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT161191 NZ_CP071086:741248-741541 [Klebsiella pneumoniae]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Structures

Toxin

• AlphaFold DB: A0A606Q844

Antitoxin

• AlphaFold DB: A0A7U9QFC4