Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3826332..3826986 Replicon chromosome
Accession NZ_CP055041
Organism Shigella flexneri strain SWHEFF_59

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag HUZ71_RS18645 Protein ID WP_000244777.1
Coordinates 3826332..3826739 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag HUZ71_RS18650 Protein ID WP_000354046.1
Coordinates 3826720..3826986 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ71_RS18625 (3822289) 3822289..3824022 - 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -
HUZ71_RS18630 (3824028) 3824028..3824738 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
HUZ71_RS18635 (3824763) 3824763..3825659 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
HUZ71_RS18640 (3825771) 3825771..3826292 + 522 WP_001055874.1 flavodoxin FldB -
HUZ71_RS18645 (3826332) 3826332..3826739 - 408 WP_000244777.1 protein YgfX Toxin
HUZ71_RS18650 (3826720) 3826720..3826986 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
HUZ71_RS18655 (3827229) 3827229..3828209 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
HUZ71_RS18660 (3828405) 3828405..3829064 - 660 WP_000250274.1 hemolysin III family protein -
HUZ71_RS18665 (3829228) 3829228..3829539 - 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
HUZ71_RS18670 (3829584) 3829584..3831017 + 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
HUZ71_RS18675 (3831074) 3831074..3831817 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T161188 WP_000244777.1 NZ_CP055041:c3826739-3826332 [Shigella flexneri]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T161188 NZ_CP071083:c6162-5857 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGACGACAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGGCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT161188 WP_000354046.1 NZ_CP055041:c3826986-3826720 [Shigella flexneri]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT161188 NZ_CP071083:c6382-6164 [Klebsiella pneumoniae]
ATGAAGCAGCGCATCACTGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References