Detailed information of TA system TA161186

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3608504..3609301
• Replicon: chromosome
• Accession: NZ_CP055041
• Organism: Shigella flexneri strain SWHEFF_59

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A346GMF9
• Locus tag: HUZ71_RS17590
• Protein ID: WP_072644276.1
• Coordinates: 3608924..3609301 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A346GRC6
• Locus tag: HUZ71_RS17585
• Protein ID: WP_072648994.1
• Coordinates: 3608504..3608878 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ71_RS17560 (3604087)	3604087..3604839	+	753	WP_000602738.1	IS6 family transposase	-
HUZ71_RS17565 (3605261)	3605261..3606286	-	1026	WP_000742814.1	aminoglycoside O-phosphotransferase APH(4)-Ia	-
HUZ71_RS17570 (3606515)	3606515..3607291	-	777	WP_001199192.1	aminoglycoside N-acetyltransferase AAC(3)-IVa	-
HUZ71_RS17575 (3607405)	3607405..3608109	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
HUZ71_RS17580 (3608204)	3608204..3608425	+	222	WP_000692353.1	DUF987 domain-containing protein	-
HUZ71_RS17585 (3608504)	3608504..3608878	+	375	WP_072648994.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HUZ71_RS17590 (3608924)	3608924..3609301	+	378	WP_072644276.1	TA system toxin CbtA family protein	Toxin
HUZ71_RS17595 (3609298)	3609298..3609465	+	168	Protein_3440	DUF5983 family protein	-
HUZ71_RS17600 (3609523)	3609523..3609720	+	198	WP_094770706.1	DUF957 domain-containing protein	-
HUZ71_RS17605 (3609817)	3609817..3610662	+	846	WP_001280435.1	DUF4942 domain-containing protein	-
HUZ71_RS17615 (3611396)	3611396..3613648	+	2253	Protein_3443	alpha-amylase family glycosyl hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	sul2 / aph(4)-Ia / aac(3)-IVa	luxS	3581204..3655884	74680

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14231.23 Da
• Isoelectric Point: 7.7761

>T161186 WP_072644276.1 NZ_CP055041:3608924-3609301 [Shigella flexneri]
MKTLPDTYVREASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMARDNYRTVNDITQGKHPEVKR

Nucleotide

• Length: 378 bp

>T161186 NZ_CP071080:c5212812-5212429 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 13869.65 Da
• Isoelectric Point: 6.2043

>AT161186 WP_072648994.1 NZ_CP055041:3608504-3608878 [Shigella flexneri]
VSDKLHETNYPDDHNDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYMAVYPTLAPATTS

Nucleotide

• Length: 375 bp

>AT161186 NZ_CP071080:c5213054-5212812 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Toxin

• AlphaFold DB: A0A346GMF9

Antitoxin

• AlphaFold DB: A0A346GRC6