Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 2374333..2374859 Replicon chromosome
Accession NZ_CP055041
Organism Shigella flexneri strain SWHEFF_59

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag HUZ71_RS11555 Protein ID WP_000323025.1
Coordinates 2374333..2374620 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag HUZ71_RS11560 Protein ID WP_000534858.1
Coordinates 2374620..2374859 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ71_RS11520 (2370306) 2370306..2370974 - 669 Protein_2247 DUF523 and DUF1722 domain-containing protein -
HUZ71_RS11525 (2371167) 2371167..2371691 + 525 WP_000780584.1 lipocalin family protein -
HUZ71_RS11530 (2371847) 2371847..2372224 - 378 WP_001204787.1 antiterminator Q family protein -
HUZ71_RS11535 (2372242) 2372242..2373291 - 1050 WP_032182205.1 DUF968 domain-containing protein -
HUZ71_RS11540 (2373293) 2373293..2373571 - 279 WP_001515373.1 hypothetical protein -
HUZ71_RS11545 (2373638) 2373638..2373889 - 252 WP_001463433.1 protein Rem -
HUZ71_RS11550 (2374106) 2374106..2374261 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
HUZ71_RS11555 (2374333) 2374333..2374620 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
HUZ71_RS11560 (2374620) 2374620..2374859 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
HUZ71_RS11565 (2374884) 2374884..2375189 + 306 WP_071593935.1 protein YdfV -
HUZ71_RS11570 (2375392) 2375392..2375724 + 333 WP_001301033.1 FlxA-like family protein -
HUZ71_RS11575 (2376161) 2376161..2377501 - 1341 WP_000589013.1 IS1202-like element ISEsa1 family transposase -
HUZ71_RS11580 (2378270) 2378270..2378556 - 287 Protein_2259 hypothetical protein -
HUZ71_RS11585 (2379034) 2379034..2379523 - 490 Protein_2260 class I SAM-dependent methyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 2341249..2404922 63673


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T161176 WP_000323025.1 NZ_CP055041:c2374620-2374333 [Shigella flexneri]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T161176 NZ_CP071076:47659-47811 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCATCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGGACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT161176 WP_000534858.1 NZ_CP055041:c2374859-2374620 [Shigella flexneri]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT161176 NZ_CP071076:c47609-47547 [Escherichia coli]
TCGAAGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References