Detailed information of TA system TA161175

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2317301..2318889
• Replicon: chromosome
• Accession: NZ_CP055041
• Organism: Shigella flexneri strain SWHEFF_59

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: HUZ71_RS11235
• Protein ID: WP_239638073.1
• Coordinates: 2317301..2318623 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: HUZ71_RS11240
• Protein ID: WP_001301023.1
• Coordinates: 2318623..2318889 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ71_RS11210 (2313267)	2313267..2314181	-	915	WP_000520676.1	fimbrial protein	-
HUZ71_RS11215 (2314240)	2314240..2314743	-	504	WP_000825452.1	fimbrial protein	-
HUZ71_RS11220 (2314756)	2314756..2315286	-	531	WP_000876763.1	fimbrial protein	-
HUZ71_RS11225 (2315300)	2315300..2316526	-	1227	Protein_2188	fimbria/pilus outer membrane usher protein	-
HUZ71_RS11230 (2316781)	2316781..2316989	-	209	Protein_2189	AraC family transcriptional regulator	-
HUZ71_RS11235 (2317301)	2317301..2318623	-	1323	WP_239638073.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
HUZ71_RS11240 (2318623)	2318623..2318889	-	267	WP_001301023.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2307290..2318889	11599

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-401)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49261.75 Da
• Isoelectric Point: 7.8725

>T161175 WP_239638073.1 NZ_CP055041:c2318623-2317301 [Shigella flexneri]
MPKLVTWMNNQRVGELTKLANGAHTFNYAPEWLASRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVTHPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGNVRALAVERFDRRW
NAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIARIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLRFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVESNVLRLHGRLSREYGSK

Nucleotide

• Length: 1323 bp

>T161175 NZ_CP071075:120852-121232 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAACCGCTACGGCGGCCTGCCGGGAATGTC
TGATCCGGGCAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGTTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGGGCTGCGACCGG
AGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10016.38 Da
• Isoelectric Point: 6.2267

>AT161175 WP_001301023.1 NZ_CP055041:c2318889-2318623 [Shigella flexneri]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTTLTTFFKILQSLELSMTLCDAKNASPES
TEQQNLEW

Nucleotide

• Length: 267 bp

>AT161175 NZ_CP071075:120631-120852 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAGGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA