Detailed information of TA system TA161174

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2232874..2233512
• Replicon: chromosome
• Accession: NZ_CP055041
• Organism: Shigella flexneri strain SWHEFF_59

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: HUZ71_RS10870
• Protein ID: WP_000813794.1
• Coordinates: 2232874..2233050 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: HUZ71_RS10875
• Protein ID: WP_001270286.1
• Coordinates: 2233096..2233512 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ71_RS10850 (2228478)	2228478..2229668	-	1191	WP_001236269.1	BenE family transporter YdcO	-
HUZ71_RS10855 (2229760)	2229760..2230296	+	537	WP_000429141.1	DNA-binding transcriptional regulator SutR	-
HUZ71_RS10860 (2230369)	2230369..2232330	+	1962	WP_032182254.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
HUZ71_RS10865 (2232422)	2232422..2232652	-	231	WP_000494244.1	YncJ family protein	-
HUZ71_RS10870 (2232874)	2232874..2233050	+	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
HUZ71_RS10875 (2233096)	2233096..2233512	+	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
HUZ71_RS10880 (2233591)	2233591..2235000	+	1410	WP_000760649.1	PLP-dependent aminotransferase family protein	-
HUZ71_RS10885 (2235242)	2235242..2236387	+	1146	WP_000047429.1	ABC transporter substrate-binding protein	-
HUZ71_RS10890 (2236405)	2236405..2237418	+	1014	WP_000220393.1	ABC transporter ATP-binding protein	-
HUZ71_RS10895 (2237419)	2237419..2238360	+	942	WP_001251319.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2227290..2228438	1148

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T161174 WP_000813794.1 NZ_CP055041:2232874-2233050 [Shigella flexneri]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T161174 NZ_CP071074:c14256-13906 [Escherichia coli]
ATGTGGGTCATCGAGACAACTGACACCTTTGATGAGTGGTTCGATGCACTGGATGATACCGATAGAGCCAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGTCCCATGCTGTCGAGACCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTGCGGGTCCAAAGCAAAGGAGATCCTATCAGGGCATTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGCGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT161174 WP_001270286.1 NZ_CP055041:2233096-2233512 [Shigella flexneri]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT161174 NZ_CP071074:c13901-13599 [Escherichia coli]
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA

Structures

Toxin

• PDB: 6HPB