Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 704944..705520 Replicon chromosome
Accession NZ_CP055041
Organism Shigella flexneri strain SWHEFF_59

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag HUZ71_RS03345 Protein ID WP_001297643.1
Coordinates 704944..705231 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag HUZ71_RS03350 Protein ID WP_000063156.1
Coordinates 705218..705520 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ71_RS03325 (701197) 701197..702477 - 1281 WP_001037382.1 DUF445 domain-containing protein -
HUZ71_RS03330 (702518) 702518..703750 - 1233 WP_001339512.1 multidrug efflux MFS transporter MdtM -
HUZ71_RS03335 (703896) 703896..703991 - 96 Protein_640 dienelactone hydrolase family protein -
HUZ71_RS03340 (704048) 704048..704745 + 698 Protein_641 IS1 family transposase -
HUZ71_RS03345 (704944) 704944..705231 + 288 WP_001297643.1 BrnT family toxin Toxin
HUZ71_RS03350 (705218) 705218..705520 + 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
HUZ71_RS03355 (705554) 705554..706510 - 957 WP_001297640.1 GTPase -
HUZ71_RS03360 (706521) 706521..706724 - 204 WP_000467859.1 YbdD/YjiX family protein -
HUZ71_RS03365 (706774) 706774..708924 - 2151 WP_001295524.1 pyruvate/proton symporter BtsT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - fimD / fimF / fimG / fimH / cheD 662539..726910 64371


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T161166 WP_001297643.1 NZ_CP055041:704944-705231 [Shigella flexneri]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T161166 NZ_CP071073:c2387039-2386761 [Escherichia coli]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAGT
CAAACGATTACGCCATCGACTTGCTGTGATTGATGCGGCAAGCTGTCTTGCTGATATCGATATGCCCGGTTACCGACTAC
ACCCGTTAAGCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGCAACTGGCGAATCACATTTGAATTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT161166 WP_000063156.1 NZ_CP055041:705218-705520 [Shigella flexneri]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT161166 NZ_CP071073:c2386761-2386477 [Escherichia coli]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCAACGGCAAGCCGATTGCTGACCGGAAAAGCAGCTTTGACGCCAGAAATGGCAA
TTAAACTTTCCGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAACGCCTGGAGTCTGGCAGAGGCTGAA
AAAACGGTGGATGTGTCGCGATTACGCCGTCTGGTTACGCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References