Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 503387..503884 Replicon chromosome
Accession NZ_CP055041
Organism Shigella flexneri strain SWHEFF_59

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag HUZ71_RS02320 Protein ID WP_001173343.1
Coordinates 503711..503884 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag HUZ71_RS02315 Protein ID WP_000398619.1
Coordinates 503387..503683 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ71_RS02295 (500118) 500118..500837 - 720 WP_000611288.1 two-component system response regulator DcuR -
HUZ71_RS02300 (500834) 500834..502465 - 1632 WP_001216477.1 sensor histidine kinase -
HUZ71_RS02305 (502646) 502646..502876 + 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
HUZ71_RS02310 (502888) 502888..503160 + 273 WP_000405647.1 GNAT family N-acetyltransferase -
HUZ71_RS02315 (503387) 503387..503683 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
HUZ71_RS02320 (503711) 503711..503884 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
HUZ71_RS02325 (504008) 504008..505525 - 1518 WP_239638030.1 lysine--tRNA ligase -
HUZ71_RS02330 (505762) 505762..507219 - 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T161164 WP_001173343.1 NZ_CP055041:503711-503884 [Shigella flexneri]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T161164 NZ_CP071073:c2160575-2160468 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT161164 WP_000398619.1 NZ_CP055041:503387-503683 [Shigella flexneri]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT161164 NZ_CP071073:2160628-2160689 [Escherichia coli]
TGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References