Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-DnaT
Location 25288..25810 Replicon plasmid unnamed2
Accession NZ_CP055039
Organism Shigella flexneri strain SWHEFF_60

Toxin (Protein)


Gene name relE Uniprot ID D0QMR1
Locus tag HUZ72_RS23900 Protein ID WP_000220561.1
Coordinates 25288..25569 (-) Length 94 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID G3CAG1
Locus tag HUZ72_RS23905 Protein ID WP_000121743.1
Coordinates 25559..25810 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ72_RS23875 (HUZ72_23680) 20311..20394 - 84 Protein_21 SOS response-associated peptidase -
HUZ72_RS23880 (HUZ72_23685) 20842..21123 + 282 WP_224732584.1 Tn3 family transposase -
HUZ72_RS23885 (HUZ72_23690) 21322..22326 - 1005 WP_000427623.1 IS110-like element IS4321 family transposase -
HUZ72_RS23890 (HUZ72_23695) 22744..24180 - 1437 WP_000105383.1 glutathione synthase -
HUZ72_RS23895 (HUZ72_23700) 24447..25151 + 705 WP_001067858.1 IS6-like element IS26 family transposase -
HUZ72_RS23900 (HUZ72_23705) 25288..25569 - 282 WP_000220561.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HUZ72_RS23905 (HUZ72_23710) 25559..25810 - 252 WP_000121743.1 plasmid stabilization protein Antitoxin
HUZ72_RS23910 (HUZ72_23715) 26686..27555 + 870 WP_001212191.1 replication initiation protein -
HUZ72_RS23915 (HUZ72_23720) 27560..28411 + 852 WP_000435059.1 replication initiation protein -
HUZ72_RS23920 (HUZ72_23725) 28415..28861 + 447 WP_001074378.1 hypothetical protein -
HUZ72_RS23925 (HUZ72_23735) 28998..29351 + 354 WP_024156393.1 DNA distortion polypeptide 3 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid dfrA12 / aadA2 / sul3 / tet(A) / qnrS1 / blaTEM-1B - 1..72259 72259


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 11023.86 Da        Isoelectric Point: 10.5938

>T161163 WP_000220561.1 NZ_CP055039:c25569-25288 [Shigella flexneri]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADMR

Download         Length: 282 bp

>T161163 NZ_CP071073:c2160040-2159933 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9194.43 Da        Isoelectric Point: 4.1683

>AT161163 WP_000121743.1 NZ_CP055039:c25810-25559 [Shigella flexneri]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Download         Length: 252 bp

>AT161163 NZ_CP071073:2160088-2160154 [Escherichia coli]
GTTCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTATACCTCTCAACGTGCGGGGGTTTTCTC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3S5I302

Antitoxin

Source ID Structure
AlphaFold DB A0A3S5I301

References