Detailed information of TA system TA161162

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 5880..6405
• Replicon: plasmid unnamed1
• Accession: NZ_CP055038
• Organism: Shigella flexneri strain SWHEFF_60

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: D4HQE7
• Locus tag: HUZ72_RS22930
• Protein ID: WP_013023785.1
• Coordinates: 5880..6185 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: W8V2V6
• Locus tag: HUZ72_RS22935
• Protein ID: WP_001568025.1
• Coordinates: 6187..6405 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ72_RS22900 (HUZ72_22710)	1567..2193	+	627	WP_004197649.1	ParA family plasmid-partitioning AAA ATPase	-
HUZ72_RS22905	2190..2492	+	303	WP_004197636.1	hypothetical protein	-
HUZ72_RS22910 (HUZ72_22720)	2956..3750	-	795	WP_004197635.1	site-specific integrase	-
HUZ72_RS22915 (HUZ72_22725)	3948..4964	-	1017	WP_017899884.1	hypothetical protein	-
HUZ72_RS22920 (HUZ72_22730)	4975..5289	-	315	WP_053389906.1	hypothetical protein	-
HUZ72_RS22925 (HUZ72_22735)	5316..5711	-	396	WP_017899885.1	hypothetical protein	-
HUZ72_RS22930 (HUZ72_22740)	5880..6185	-	306	WP_013023785.1	type II toxin-antitoxin system toxin CcdB	Toxin
HUZ72_RS22935 (HUZ72_22745)	6187..6405	-	219	WP_001568025.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
HUZ72_RS22940 (HUZ72_22750)	7223..7513	-	291	WP_013023783.1	hypothetical protein	-
HUZ72_RS22945 (HUZ72_22755)	7510..8637	-	1128	WP_013023782.1	DUF4238 domain-containing protein	-
HUZ72_RS22950 (HUZ72_22760)	8671..10194	-	1524	WP_017899887.1	hypothetical protein	-
HUZ72_RS22955 (HUZ72_22765)	10470..11249	-	780	WP_013023780.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sul2 / aph(3'')-Ib / aph(6)-Id / aph(3')-Ia / aac(3)-IId / aac(6')-Ib-cr / ARR-3 / dfrA27 / aadA16 / qacE / sul1 / qnrB91 / mph(A) / tet(A) / floR / blaTEM-1B / blaCTX-M-3 / qnrS1	-	1..140589	140589

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11615.30 Da
• Isoelectric Point: 6.4672

>T161162 WP_013023785.1 NZ_CP055038:c6185-5880 [Shigella flexneri]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T161162 NZ_CP071073:c1943218-1942724 [Escherichia coli]
ATGATTCAGGCCCCCGAGCCACTCATGGCAAGGCATCAATTCACCTCTTTTTGCTCGGGAGTAGAAACGATGGATAACTG
GCTAAAGCAGAGAGCCCTAAAAAATCAGCTTGCCGGCGCATCACGTACATTTGTTAGCTGTGATACGTATAGCAACGTTT
TGGCATATTATTCGCTTGCTTCCAGCGCCGTTGAAACGTATGTCGCGACAGGACGATTTCGCCGAAATATGCCAGAACCG
ATCCCTGTTGTAGTACTTGGTCGTCTGGCAATAGATAAATCATTACAGGGTCAGGGCATCGGTCGTGCGATGGTCAGAGA
TGCCGGTCTACGAGTACTACAGGCGGCTGAAGTCATTGGTATTCGCGGAATGTTGGTCCATGCGTTATCAGACCAGGCCC
GTGAGTTTTATCTGCGAGTGGGGTTTGAACCATCGCCTGTCGATTCTATGATACTGATGGCAACATTGGCTGATTTGCAG
GAATGTCTTAAATAG

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8392.34 Da
• Isoelectric Point: 4.7405

>AT161162 WP_001568025.1 NZ_CP055038:c6405-6187 [Shigella flexneri]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT161162 NZ_CP071073:c1943508-1943215 [Escherichia coli]
ATGCCAGCAGCCAATAACATTCCAACCAGGCGCGAATCTTTAAATTTGCGCATAAAACCCGCAGAACGCGATCTTATTGA
CCGGGCCGCTAAAGCCCGAGGAAAAAATCGCACCGAATTTGTCCTTGAGGCCGCCAGAGCCGCAGCTGAAGAGGCGCTCA
TTGAGCAGAGAATTTTCAGGATCGATTCTGATGATTACCAGCTATTTTTGAATCGTCTGGATCAGCCCCCCGCGCCCAAT
GCTGCATTACGCAAAACCATGCAAACACCCGCGCCGTGGGAGCAAAAAAAATGA

Structures

Toxin

• AlphaFold DB: A0A2J4ZZW6

Antitoxin

• AlphaFold DB: A0A2J4ZZP8