Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpSB/PRK09812-ChpS
Location 4164311..4164906 Replicon chromosome
Accession NZ_CP055037
Organism Shigella flexneri strain SWHEFF_60

Toxin (Protein)


Gene name chpB Uniprot ID U9Y4M4
Locus tag HUZ72_RS20245 Protein ID WP_000239579.1
Coordinates 4164311..4164661 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name chpS Uniprot ID U9Y2K1
Locus tag HUZ72_RS20250 Protein ID WP_001223208.1
Coordinates 4164655..4164906 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ72_RS20225 (4159757) 4159757..4160779 - 1023 WP_001295197.1 ABC transporter permease -
HUZ72_RS20230 (4160793) 4160793..4162295 - 1503 WP_000205784.1 sugar ABC transporter ATP-binding protein -
HUZ72_RS20235 (4162435) 4162435..4163391 - 957 WP_000265933.1 galactofuranose ABC transporter substrate-binding protein YtfQ -
HUZ72_RS20240 (4163701) 4163701..4164231 + 531 WP_000055072.1 inorganic diphosphatase -
HUZ72_RS20245 (4164311) 4164311..4164661 - 351 WP_000239579.1 endoribonuclease toxin ChpB Toxin
HUZ72_RS20250 (4164655) 4164655..4164906 - 252 WP_001223208.1 type II toxin-antitoxin system antitoxin ChpS Antitoxin
HUZ72_RS20255 (4165118) 4165118..4165459 - 342 WP_001219160.1 gamma-glutamylcyclotransferase -
HUZ72_RS20260 (4165462) 4165462..4169241 - 3780 WP_000060901.1 autotransporter assembly complex protein TamB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-114)

Antitoxin

SpoVT-AbrB domain

(6-49)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12511.45 Da        Isoelectric Point: 5.6219

>T161160 WP_000239579.1 NZ_CP055037:c4164661-4164311 [Shigella flexneri]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Download         Length: 351 bp

>T161160 NZ_CP071073:c1203274-1203056 [Escherichia coli]
ATGTCCGAAAAACCTTTAACAAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9271.58 Da        Isoelectric Point: 4.4201

>AT161160 WP_001223208.1 NZ_CP055037:c4164906-4164655 [Shigella flexneri]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Download         Length: 252 bp

>AT161160 NZ_CP071073:c1203674-1203300 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y8A8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LQ26

References