Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3552700..3553318 Replicon chromosome
Accession NZ_CP055037
Organism Shigella flexneri strain SWHEFF_60

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag HUZ72_RS17305 Protein ID WP_001291435.1
Coordinates 3553100..3553318 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag HUZ72_RS17300 Protein ID WP_000344800.1
Coordinates 3552700..3553074 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ72_RS17290 (3547789) 3547789..3548982 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
HUZ72_RS17295 (3549005) 3549005..3552154 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
HUZ72_RS17300 (3552700) 3552700..3553074 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
HUZ72_RS17305 (3553100) 3553100..3553318 + 219 WP_001291435.1 HHA domain-containing protein Toxin
HUZ72_RS17310 (3553490) 3553490..3554041 + 552 WP_000102564.1 maltose O-acetyltransferase -
HUZ72_RS17315 (3554157) 3554157..3554627 + 471 WP_000136192.1 YlaC family protein -
HUZ72_RS17320 (3554791) 3554791..3555183 + 393 Protein_3401 cyclic-guanylate-specific phosphodiesterase PdeB -
HUZ72_RS17330 (3555952) 3555952..3557117 + 1166 Protein_3403 cyclic-guanylate-specific phosphodiesterase PdeB -
HUZ72_RS17335 (3557159) 3557159..3557491 - 333 WP_001741293.1 DUF1428 family protein -
HUZ72_RS17345 (3557891) 3557891..3558202 + 312 WP_000409911.1 MGMT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T161155 WP_001291435.1 NZ_CP055037:3553100-3553318 [Shigella flexneri]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T161155 NZ_CP071073:314756-314929 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTAGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT161155 WP_000344800.1 NZ_CP055037:3552700-3553074 [Shigella flexneri]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT161155 NZ_CP071073:314432-314728 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACCGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References