Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 913577..914231 Replicon chromosome
Accession NZ_CP055037
Organism Shigella flexneri strain SWHEFF_60

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag HUZ72_RS04405 Protein ID WP_000244777.1
Coordinates 913824..914231 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag HUZ72_RS04400 Protein ID WP_000354046.1
Coordinates 913577..913843 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ72_RS04375 (908746) 908746..909489 + 744 WP_239637467.1 SDR family oxidoreductase -
HUZ72_RS04380 (909546) 909546..910979 - 1434 WP_001338826.1 6-phospho-beta-glucosidase BglA -
HUZ72_RS04385 (911024) 911024..911335 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
HUZ72_RS04390 (911499) 911499..912158 + 660 WP_000250274.1 hemolysin III family protein -
HUZ72_RS04395 (912354) 912354..913334 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
HUZ72_RS04400 (913577) 913577..913843 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
HUZ72_RS04405 (913824) 913824..914231 + 408 WP_000244777.1 protein YgfX Toxin
HUZ72_RS04410 (914271) 914271..914792 - 522 WP_001055874.1 flavodoxin FldB -
HUZ72_RS04415 (914904) 914904..915800 + 897 WP_000806639.1 site-specific tyrosine recombinase XerD -
HUZ72_RS04420 (915825) 915825..916535 + 711 WP_000715216.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
HUZ72_RS04425 (916541) 916541..918274 + 1734 WP_000813212.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T161141 WP_000244777.1 NZ_CP055037:913824-914231 [Shigella flexneri]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T161141 NZ_CP071063:c3869810-3869400 [Enterobacter mori]
GTGGTCCTGTGGCAATCTGATCTTCGCGTCTCATGGCGCTCGCAGTGGATGTCATTACTGCTCCACGGCCTGGTTGCGGC
CTTTGTTTTACTGATGCCGTGGCCGCTAAGCTATACGCCTCTGTGGTTATTGCTGCTGTCGTTTGTGGTTTTTGATAGCG
TGCGCAGCCAGCGCCGGATCAACGCCCGTCAGGGTGAAATCAAACTGCTGGTGGATTCCCGTCTGCGCTGGCAGGGGAAA
GAGTGGGAGATCGTCGGTACACCGTGGATGCTTAGCGTTGGCATGATGCTGAGACTGCGTCAGCCTGAAGGTGGTCGTCG
CCAGCACCTGTGGCTGGCTGCCGACAGTATGGATGCTGCGGAGTGGCGAGATCTGCGCCGGATGATGTTGCAACAACCGA
CGCAGGACTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT161141 WP_000354046.1 NZ_CP055037:913577-913843 [Shigella flexneri]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT161141 NZ_CP071063:c3870057-3869791 [Enterobacter mori]
ATGGATATTAACAACAAGGCCCGTATTCACTGGGCGTGCCGTCGCGGCATGCGCGAACTTGATATCTCCATCATGCCGTT
CTTTGAACATGAGTATGACAGCTTAAGCGATGACGATAAGCGCCTGTTTGTTCGCCTTCTTGAGTCTGACGATCCGGATT
TATTCAACTGGCTGATGAATCACGGCAAACCCGCCGACACCGAGTTGCAACGGATGGTGCAATTAATTCAAACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References