Detailed information of TA system TA161140

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 786317..787088
• Replicon: chromosome
• Accession: NZ_CP055037
• Organism: Shigella flexneri strain SWHEFF_60

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: HUZ72_RS03830
• Protein ID: WP_000854727.1
• Coordinates: 786317..786694 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: HUZ72_RS03835
• Protein ID: WP_239637542.1
• Coordinates: 786783..787088 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ72_RS03800 (781725)	781725..782702	+	978	WP_000633238.1	type II secretion system minor pseudopilin GspK	-
HUZ72_RS03805 (782699)	782699..783877	+	1179	WP_000094976.1	type II secretion system protein GspL	-
HUZ72_RS03810 (783879)	783879..784415	+	537	WP_000942792.1	GspM family type II secretion system protein YghD	-
HUZ72_RS03815 (784696)	784696..785538	-	843	WP_239637462.1	DUF4942 domain-containing protein	-
HUZ72_RS03820 (785623)	785623..785820	-	198	WP_089613747.1	DUF957 domain-containing protein	-
HUZ72_RS03825 (785832)	785832..786320	-	489	WP_089613746.1	DUF5983 family protein	-
HUZ72_RS03830 (786317)	786317..786694	-	378	WP_000854727.1	TA system toxin CbtA family protein	Toxin
HUZ72_RS03835 (786783)	786783..787088	-	306	WP_239637542.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HUZ72_RS03840 (787230)	787230..787451	-	222	WP_000692323.1	DUF987 domain-containing protein	-
HUZ72_RS03845 (787520)	787520..787996	-	477	WP_143359950.1	RadC family protein	-
HUZ72_RS03850 (788012)	788012..788497	-	486	WP_000214415.1	antirestriction protein	-
HUZ72_RS03855 (788589)	788589..789407	-	819	WP_237441638.1	DUF932 domain-containing protein	-
HUZ72_RS03860 (789506)	789506..789739	-	234	WP_001278281.1	DUF905 family protein	-
HUZ72_RS03865 (789745)	789745..790422	-	678	WP_053291273.1	hypothetical protein	-
HUZ72_RS03870 (790541)	790541..791425	-	885	WP_053291272.1	50S ribosome-binding GTPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (2-96)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14345.39 Da
• Isoelectric Point: 9.2442

>T161140 WP_000854727.1 NZ_CP055037:c786694-786317 [Shigella flexneri]
MKTLPDTHVREASRCPSPVTIWQTLLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPRF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITRGKHPEAKR

Nucleotide

• Length: 378 bp

>T161140 NZ_CP071063:3612653-3612952 [Enterobacter mori]
ATGATTAATCATTTTCGCGATCAATGGCTTATGGATTTTTTCCTCTTTGGAAAATCAAGCAACGTTATCCCATCAAGCCT
TGAGGCAGCCCTGGCGAGGAAGCTGGATATCATCAATGCAGCATCCTCATATCGGGATTTGCGATCCCCACCAGGTAACA
TGTATGAATCGTTGAATCCTCCACTGGAAGGATATTCCTCAATTCGCGTGAACAGGCAATACAGATTGGTATTCAGATGG
ACTGAGGGCAAAGCGGACGATCTCTACCTCTCTCCCCACAAATACACATCGCACAAATGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11295.97 Da
• Isoelectric Point: 8.1351

>AT161140 WP_239637542.1 NZ_CP055037:c787088-786783 [Shigella flexneri]
MPCTVTPCFGARLVQESNQLHYLANRAGIRGRFSDADAYHLDQTFPLLMKQLELMLTSGELNPRHQHTVTLYARGLTCKA
DTLGSCGYVYLAVYPTSETKK

Nucleotide

• Length: 306 bp

>AT161140 NZ_CP071063:3612963-3613310 [Enterobacter mori]
ATGACCCTTCAACAAGCTCTGAGAAAACCCACCACGCCGGGTGACGTGCTGCAGTATGAGTATCTTGAACCGCTAAATCT
CAAAATTAGCGATCTGGCGGAGATGCTTAATGTACACCGCAATACCATCAGCGCATTGGTCAATAACAACCGCAAGCTTA
CTGCCGATATGGCGATCAAACTGGCAAAAGCCTTTGATACCACTATTGAATTTTGGCTGAACTTGCAACTGAACGTCGAT
ATCTGGGAAGCGCAATCTAACGCCAGGACGCAGGAGGAGTTGAGCCGTATAAAGACGGTGGTGGAAATAATGGCTAAAAG
GAAATCCGGTCAGCCGGATGTGGCCTGA