Detailed information of TA system TA161134

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 54899..55542
• Replicon: plasmid unnamed1
• Accession: NZ_CP055034
• Organism: Klebsiella pneumoniae strain SWHEFF_62

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: HUZ73_RS25855
• Protein ID: WP_016236302.1
• Coordinates: 55126..55542 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D5KTK7
• Locus tag: HUZ73_RS25850
• Protein ID: WP_001261282.1
• Coordinates: 54899..55129 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ73_RS25820 (HUZ73_25735)	49908..51083	+	1176	Protein_48	IS3 family transposase	-
HUZ73_RS25825 (HUZ73_25740)	51465..52250	-	786	WP_050485696.1	site-specific integrase	-
HUZ73_RS25830 (HUZ73_25745)	52296..52817	-	522	WP_032448294.1	hypothetical protein	-
HUZ73_RS25835 (HUZ73_25750)	52875..53267	-	393	WP_074422814.1	hypothetical protein	-
HUZ73_RS25840 (HUZ73_25755)	53376..54320	-	945	WP_032448293.1	hypothetical protein	-
HUZ73_RS25845 (HUZ73_25760)	54520..54942	-	423	WP_050485703.1	hypothetical protein	-
HUZ73_RS25850 (HUZ73_25765)	54899..55129	+	231	WP_001261282.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
HUZ73_RS25855 (HUZ73_25770)	55126..55542	+	417	WP_016236302.1	type II toxin-antitoxin system VapC family toxin	Toxin
HUZ73_RS25860 (HUZ73_25775)	55616..57178	+	1563	WP_004206609.1	AAA family ATPase	-
HUZ73_RS25865 (HUZ73_25780)	57163..58185	+	1023	WP_000361404.1	helicase UvrD	-
HUZ73_RS25870 (HUZ73_25785)	58730..59638	+	909	WP_239594585.1	HNH endonuclease	-
HUZ73_RS25875 (HUZ73_25790)	59824..60174	-	351	WP_004187110.1	DUF305 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	qnrS1 / blaLAP-2 / aph(6)-Id / floR / sul2 / aph(4)-Ia / aac(3)-IVa / aph(3')-Ia / aadA2 / dfrA12 / tet(A)	iutA / iucD / iucC / iucB / iucA / pla	1..242129	242129

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15091.55 Da
• Isoelectric Point: 7.1084

>T161134 WP_016236302.1 NZ_CP055034:55126-55542 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLELAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Nucleotide

• Length: 417 bp

>T161134 NZ_CP071062:4913656-4914975 [Dickeya solani]
ATGGCGGCGCTCGATGTTTACATGAACGGCTACCGGGTGGGAATTCTGACCAAAACGGGCAGTGGCGCGCATCATTTTTC
CTATGATGCGAACTGGCTCGAGTTGCCGGGGAGTCGCCCCCTCTCGCTCTCAATGCCGCTACGCCATCAGCCCTATCAAG
GTGATGAAGTTTATAATTTCTTCGACAACTTGTTGCCGGATAACCCGGATATCAGGCGGCGGATTGTCACCAGGCATCAG
GCGGATTCCACCCAGCCGTTTGATTTGCTGGCTAAAGTGGGGCAAGACAGTGTTGGGGCGTTGCAATTGGTGCCGCAGGG
CACCGCTGTTGGCGATATAAAACAGATCGAATACAAAACCTTATCCGAGCAACAGCTTGAAAACATACTGGCGGGATATC
TGTCCGATGCCCCGCTCGGGATGATTGATACAGAAGAGGATTTTCGTATCTCTATCGCAGGCGCACAGGAAAAGACGGCG
CTGCTTTATCTGGATGGCAGCTGGTGTTTGCCTCTTAATACCACACCAACTACCCACATCATCAAGCTACCGATTGGTAA
AATTGAGAGTCATTCATACTCAATTGATATGTCTGACAGCGTTGAAAATGAATACCTGTGCCTGCTCATTGCCAAGGCGT
TTGGGCTGTCGGTACCGCACTGTTTTATGATCACAGCAGGCAAGATAAAAGCGCTGGCGGTAGAACGTTTTGATCGTAAA
TACGCCTCGGATGGTCGTTGGATTATGCGGTTGCCGCAGGAGGATTTTTGCCAGGTATTAAACGTGCCTTCCGCACGAAA
ATACGAAAATCATGGTGGACCGGGCATTTCCGCTATTATGTCGGTGCTATTAGGGGCGATTGAACCTGAAAGGGATCGCT
ATACCTTTATGAAGGCGCAGGTGCTGTTTTGGCTATTGGCTGCAACGGATGGACATGCAAAAAACTTCTCCCTGTTTATT
GAATTTGAAGGACGATATCGCCTCACGCCTTTCTATGACATTCTCTCTATGTACCCGGCGATGGGCGGGCGGGGAATTGA
CCGCAGAGAGGCCAAACTGGCGATGGGGCTGACCGGCTCAAAAGGCAAGAAATACGCCATTGAGCAGATCTTTCCACGGC
ACTTTTTCCAAACGGCAAAGGTGGTTGGATTTGCCAGAGAATCGATGGAACGCATTCTGACTGAATTTTCACAGACGGTT
GATTCTGTCATCGTGAGTGTCAGAAACCAACTGCCTGCGGATTTTCCCACTCCTATCCGCGATGCGATCCTGGGAGGGCT
ACAGACTAGATCGAGTCGATTGACGACGGGCTGGGAATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8756.83 Da
• Isoelectric Point: 4.4692

>AT161134 WP_001261282.1 NZ_CP055034:54899-55129 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT161134 NZ_CP071062:4913387-4913656 [Dickeya solani]
ATGAAGGTCACCAACAGCAAACAGCTTAGCAGCTATCTAAAAGATGTTCGTATCACGCAGAAGCTTTCCCAAGGGAAAGT
GGCCAGTAAAGTGGGGATTCGTCAGGACACGGTATCGAGCTTCGAACAACATCCCGATTCCACCAAGCTGGAAACCTTCT
TTAAGATCCTGTCGGCATTAAATTTAGAACTTACCATTACCCCCAGAAATGCGCATGCAGCCAATAACGAGGCTTCTGTT
GCAACATCAGCCTGGAAGGAAGAATGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A0H3GZG3