Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5147758..5148383 Replicon chromosome
Accession NZ_CP055033
Organism Klebsiella pneumoniae strain SWHEFF_62

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag HUZ73_RS25210 Protein ID WP_002882817.1
Coordinates 5147758..5148141 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag HUZ73_RS25215 Protein ID WP_004150355.1
Coordinates 5148141..5148383 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ73_RS25195 (5145124) 5145124..5146026 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
HUZ73_RS25200 (5146023) 5146023..5146658 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
HUZ73_RS25205 (5146655) 5146655..5147584 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
HUZ73_RS25210 (5147758) 5147758..5148141 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
HUZ73_RS25215 (5148141) 5148141..5148383 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
HUZ73_RS25220 (5148588) 5148588..5149505 + 918 WP_023302328.1 alpha/beta hydrolase -
HUZ73_RS25225 (5149519) 5149519..5150460 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
HUZ73_RS25230 (5150505) 5150505..5150942 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
HUZ73_RS25235 (5150939) 5150939..5151799 - 861 WP_004146232.1 virulence factor BrkB family protein -
HUZ73_RS25240 (5151793) 5151793..5152392 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T161133 WP_002882817.1 NZ_CP055033:c5148141-5147758 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T161133 NZ_CP071062:c2717190-2716813 [Dickeya solani]
ATGAAATGGGTGAGTGCGCAGGACGTCATTGACTTCCATGATCGTATTTTGCAGGTGTTGCCTGGTGTGGTGGGTATGGC
TGATCCGGGTCGGGCTGAGGAGTTGATTTATCGAGTACAAAACCGTCTTTATTATGAAGGTGTGACCGAACTGTTTGAGT
TGGCGGCGACGTATTGGGTAACTATTGCTCGTGGTCATATTTTTAATGATGGCAATAAACGCACTGCTTTTTTTGTTACC
ATGACATTTTTGCGCCGCAATGGCATATTGATTGTCGATAACGATAATTCGCTGGAAGAACTCACCATTAAGGCGGCAAC
CGGAGAATGCCTGGTGTCGGAACTCGCCGAACAATTAAGACAACGGGTGGAAAAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT161133 WP_004150355.1 NZ_CP055033:c5148383-5148141 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT161133 NZ_CP071062:c2717408-2717187 [Dickeya solani]
ATGAAGACTTATACCATTACTGAAGCCCGGCAAAATATTGCGACAGTCATTGATACGGCGGCCAGCGGTGAGCCGGTAGA
GATTAAGCGTCGGGATGGCACCTCGGCTGTACTCATCCCCAAAGCAGAGTTTGAGGCTTATCAGAAAGCCAGATTAGACG
CTGAATTCGATTTCATCATGCAGCGCCACGGACACACAATCAAAGCGCTCGCCGACAGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References