Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4561521..4562331 Replicon chromosome
Accession NZ_CP055033
Organism Klebsiella pneumoniae strain SWHEFF_62

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag HUZ73_RS22470 Protein ID WP_004178461.1
Coordinates 4561521..4562054 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag HUZ73_RS22475 Protein ID WP_002887278.1
Coordinates 4562065..4562331 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ73_RS22465 (4560352) 4560352..4561473 + 1122 WP_012737335.1 cupin domain-containing protein -
HUZ73_RS22470 (4561521) 4561521..4562054 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
HUZ73_RS22475 (4562065) 4562065..4562331 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
HUZ73_RS22480 (4562434) 4562434..4563867 - 1434 WP_029498528.1 Cu(+)/Ag(+) sensor histidine kinase -
HUZ73_RS22485 (4563857) 4563857..4564540 - 684 WP_023302433.1 copper response regulator transcription factor CusR -
HUZ73_RS22490 (4564712) 4564712..4566097 + 1386 WP_023302432.1 efflux transporter outer membrane subunit -
HUZ73_RS22495 (4566115) 4566115..4566459 + 345 WP_004214146.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T161129 WP_004178461.1 NZ_CP055033:c4562054-4561521 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T161129 NZ_CP071062:c1768117-1767938 [Dickeya solani]
ATGGACAGCAGGACGCTAATAGCAGAAATCAAGGCCGATGGATGGGAACTGGTAAGGGTTAACGGCAGTCATCACCATTT
TACCCACCCAACCAAACCGGGATTAGTGACCATCCCACACCCGAAGAAGGATTTACCGATAGGTACGGTAAAAAGCATCA
GGAAACAAGCGGGAATATGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT161129 WP_002887278.1 NZ_CP055033:c4562331-4562065 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT161129 NZ_CP071062:c1767910-1767500 [Dickeya solani]
ATGTTTTATCCCATTGCAATTGAAGCAGGCGACGATACTCATGCCTACGGCGTGACAGTGCCGGATCTGCCGGGCTGTTT
CTCCGCAGGTGATACGCTTGATGAGGCCATCGCCAATGCCAAAGACGCGATCACCGGCCATATCGAGCTGCTGATCGAGA
TGGGTCAGGATATTCCTGCTGTGTCATCAGTCGGAACGCTGGCGAAAGCACCGGAGTACACCGGTTACACCTGGGCTGTT
GTTGATATCGATGTCACCCGGCTGATGGGTGGGGCAGAGAAAATTAATGTCACGTTGCCGAAATCGCTGATTGACCGTAT
TGACCGTTGCGTAGCGAGTAACCCGGAATTTAAGAGCCGCTCAGGGTTCCTTGCTCAGGCAGCGTTAGAGCGGATTTCAT
CCATTCGCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References