Detailed information of TA system TA161127

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2527025..2528613
• Replicon: chromosome
• Accession: NZ_CP055033
• Organism: Klebsiella pneumoniae strain SWHEFF_62

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A486S9S0
• Locus tag: HUZ73_RS12440
• Protein ID: WP_023301940.1
• Coordinates: 2527025..2528347 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: HUZ73_RS12445
• Protein ID: WP_024622638.1
• Coordinates: 2528347..2528613 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ73_RS12425 (2522081)	2522081..2524582	+	2502	WP_023342811.1	fimbria/pilus outer membrane usher protein	-
HUZ73_RS12430 (2524570)	2524570..2525574	+	1005	WP_023301939.1	fimbrial protein	-
HUZ73_RS12435 (2525716)	2525716..2527008	+	1293	WP_002904794.1	glycoside hydrolase family 10 protein	-
HUZ73_RS12440 (2527025)	2527025..2528347	-	1323	WP_023301940.1	type II toxin-antitoxin system HipA family toxin	Toxin
HUZ73_RS12445 (2528347)	2528347..2528613	-	267	WP_024622638.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
HUZ73_RS12450 (2528878)	2528878..2529204	-	327	WP_004184023.1	hydroxyisourate hydrolase	-
HUZ73_RS12455 (2529201)	2529201..2529701	-	501	WP_004184022.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
HUZ73_RS12460 (2529679)	2529679..2531058	-	1380	WP_023301941.1	nucleobase:cation symporter-2 family protein	-
HUZ73_RS12465 (2531404)	2531404..2532558	+	1155	WP_002904783.1	FAD-dependent urate hydroxylase HpxO	-
HUZ73_RS12470 (2532539)	2532539..2533462	-	924	WP_002904781.1	LysR family hpxDE operon transcriptional regulator HpxR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (17-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49185.49 Da
• Isoelectric Point: 7.5358

>T161127 WP_023301940.1 NZ_CP055033:c2528347-2527025 [Klebsiella pneumoniae]
MATLTTWMNNVRVGTLTRQANGAHSFRYEEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITSQDDFRISVAGAQEKTAL
LRMGEQWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARELGLAVPEAEIITTPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILQEFAGHVP
QAIESARRTLPADFSSHVWQAITENMLKLHARLQQGLQAL

Nucleotide

• Length: 1323 bp

>T161127 NZ_CP071062:c1222673-1222470 [Dickeya solani]
ATGAAAAAAATCGACTATTTAATGCGTTTGCGTAAATGCACAACCATCGACACGCTTGAGCGTGTTATAGAAAAAAACAA
GTACGAGCTTTCCAACGATGAGCTTGAAATGTTCTATTCAGCTGCAGATCACCGCCTGGCAGAGCTGACGATGAATAAGC
TTTACGACAAAGTACCGACTGCTGTGTGGAGATACGTTCGCTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9924.30 Da
• Isoelectric Point: 8.5912

>AT161127 WP_024622638.1 NZ_CP055033:c2528613-2528347 [Klebsiella pneumoniae]
MNSPQIYSPVQLANYMKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKAAETAPAP
DNNTDLDW

Nucleotide

• Length: 267 bp

>AT161127 NZ_CP071062:c1223095-1222727 [Dickeya solani]
ATGGATGAGTACACACCCCAACATTATGATATTGCCCAACTCAGATTCTTGTGTGAAAATCTGCACGACGAAAGTATCGC
GACATTAGGTGACAGCAGTCATGGCTGGGTGAATGATCCGACCTCTGCGGTCAACCTTCAACTAAACGAACTTATCGAGC
ATATTGCGGCATTTGTTGTTACCTACAAAATAAAATACCCGCATGAAGCGGCGCTATGTGAACGGGTTGAGAAATATCTG
GACGACACTTATATTCTTTTCAGCAATTACGGTATCAATGACACCGAGTTGCGGAAATGGCAAAAATCCAAGTCACAATT
ATTCAGAATGTTCTCAGAAAAGAGCATCTGTACGGTAGTAAAAACTTAA

Structures

Toxin

• AlphaFold DB: A0A486S9S0