Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4240112..4240609 Replicon chromosome
Accession NZ_CP055030
Organism Shigella flexneri strain SWHEFF_64

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag HUZ74_RS20660 Protein ID WP_001173343.1
Coordinates 4240112..4240285 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag HUZ74_RS20665 Protein ID WP_000398619.1
Coordinates 4240313..4240609 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ74_RS20650 (4236782) 4236782..4238239 + 1458 WP_000856841.1 dipeptide/tripeptide permease DtpC -
HUZ74_RS20655 (4238476) 4238476..4239993 + 1518 WP_001295074.1 lysine--tRNA ligase -
HUZ74_RS20660 (4240112) 4240112..4240285 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
HUZ74_RS20665 (4240313) 4240313..4240609 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
HUZ74_RS20670 (4240836) 4240836..4241108 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
HUZ74_RS20675 (4241120) 4241120..4241350 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
HUZ74_RS20680 (4241531) 4241531..4243162 + 1632 WP_124846237.1 sensor histidine kinase -
HUZ74_RS20685 (4243159) 4243159..4243878 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T161116 WP_001173343.1 NZ_CP055030:c4240285-4240112 [Shigella flexneri]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T161116 NZ_CP071042:908220-908570 [Bacillus amyloliquefaciens]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAGAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT161116 WP_000398619.1 NZ_CP055030:c4240609-4240313 [Shigella flexneri]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT161116 NZ_CP071042:907933-908214 [Bacillus amyloliquefaciens]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References