Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 3749221..3750008 Replicon chromosome
Accession NZ_CP055030
Organism Shigella flexneri strain SWHEFF_64

Toxin (Protein)


Gene name yeeV Uniprot ID A0A377E3D6
Locus tag HUZ74_RS18280 Protein ID WP_001194695.1
Coordinates 3749631..3750008 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A2H9G3E7
Locus tag HUZ74_RS18275 Protein ID WP_000066236.1
Coordinates 3749221..3749580 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ74_RS18230 (3744461) 3744461..3744913 + 453 WP_001020418.1 hypothetical protein -
HUZ74_RS18235 (3744910) 3744910..3745362 + 453 WP_000734138.1 hypothetical protein -
HUZ74_RS18240 (3745425) 3745425..3745967 + 543 WP_001104018.1 DUF4339 domain-containing protein -
HUZ74_RS18245 (3746027) 3746027..3746479 + 453 WP_001061894.1 IrmA family protein -
HUZ74_RS18250 (3746556) 3746556..3746789 + 234 WP_001114682.1 DUF905 domain-containing protein -
HUZ74_RS18255 (3746909) 3746909..3747727 + 819 WP_124846291.1 DUF932 domain-containing protein -
HUZ74_RS18260 (3747995) 3747995..3748464 + 470 Protein_3591 antirestriction protein -
HUZ74_RS18265 (3748476) 3748476..3748955 + 480 WP_000437750.1 DNA repair protein RadC -
HUZ74_RS18270 (3748976) 3748976..3749197 + 222 WP_000691981.1 DUF987 domain-containing protein -
HUZ74_RS18275 (3749221) 3749221..3749580 + 360 WP_000066236.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
HUZ74_RS18280 (3749631) 3749631..3750008 + 378 WP_001194695.1 TA system toxin CbtA family protein Toxin
HUZ74_RS18285 (3750005) 3750005..3750496 + 492 WP_000777682.1 DUF5983 family protein -
HUZ74_RS18290 (3750528) 3750528..3750731 + 204 WP_000413747.1 DUF957 domain-containing protein -
HUZ74_RS18295 (3750812) 3750812..3751656 + 845 Protein_3598 DUF4942 domain-containing protein -
HUZ74_RS18305 (3751998) 3751998..3752729 - 732 WP_001340895.1 DNA polymerase III subunit epsilon -
HUZ74_RS18310 (3752794) 3752794..3753261 + 468 WP_000917883.1 ribonuclease HI -
HUZ74_RS18315 (3753258) 3753258..3753980 - 723 WP_001326702.1 class I SAM-dependent methyltransferase -
HUZ74_RS18320 (3754014) 3754014..3754769 + 756 WP_001052715.1 hydroxyacylglutathione hydrolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(15-114)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14180.97 Da        Isoelectric Point: 7.4209

>T161114 WP_001194695.1 NZ_CP055030:3749631-3750008 [Shigella flexneri]
MQTQPLSSTQEATSRPSPVEIWQRLLSHLLDRHYGLTLNDTPFGNDGVIQEHIDAGISLCDAVNFIVEKYDLVRIDRHGF
STETQLPRLTSIDILRARKATGLMTRNDYRTVTDITTGKYRGGHR

Download         Length: 378 bp

>T161114 NZ_CP071028:258981-259265 [Klebsiella pneumoniae]
ATGACCTATAAGCTGGCCTTTAATGAATCAGCTCTGAAAGAATGGAAAAAATTGGGCCATACCATTCGGGAACAATTCAA
AAAGAAGCTCGCTGAGCGCCTGTTGAACCCACGGGTGCCCGCATCGCAACTCCATGGACGCAAAGACCAGTACAAAATAA
AGCTCCGTGGCGCAGGCTACCGACTGGTTTACAGTGTCAACGATGATGTTGTAACGGTCACCGTAATTGGCGTCGGTAAA
CGTGAGAATGATGATATTTACAATTTGACCAAACACAGAAATTAA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13127.02 Da        Isoelectric Point: 7.0261

>AT161114 WP_000066236.1 NZ_CP055030:3749221-3749580 [Shigella flexneri]
MSNKIPTVNHDITEPWWGLKRSITPCFGARLVQSGNCLHYLADRASITGQFSDGDLRHLDQAFPLLLKQLELMLTSGELT
PRHQHCVTLYAKGLTCEADSLGSHGYVYLAIYPTPAATA

Download         Length: 360 bp

>AT161114 NZ_CP071028:258743-258991 [Klebsiella pneumoniae]
ATGGCATTTCAGGTACTAACCACTACTGCAGCAAGCATCACAGAGCTTAAACGTGATCCTATGGGCACCTTCAATGCAGG
AGAAGGTGCCCCGGTAGCCATACTGAATCGCAACGAACCCGCTTTTTACTGTGTACCGCCTGCTCTGTATGCTCAACTGA
TGGAGCTTCTTGAAGATCAGGAGCTGGGTCATATTGCTGATGAGCGAATTGGTGAGGCGGTAATCGAGGTCAGTCTAGAT
GACCTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A377E3D6

Antitoxin

Source ID Structure
AlphaFold DB A0A2H9G3E7

References