Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 3566868..3567511 Replicon chromosome
Accession NZ_CP055030
Organism Shigella flexneri strain SWHEFF_64

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag HUZ74_RS17310 Protein ID WP_001044768.1
Coordinates 3566868..3567284 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag HUZ74_RS17315 Protein ID WP_001261287.1
Coordinates 3567281..3567511 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ74_RS17285 (3562268) 3562268..3562591 + 324 WP_001175593.1 metalloregulator ArsR/SmtB family transcription factor -
HUZ74_RS17290 (3562692) 3562692..3563402 + 711 WP_057102336.1 arsenical resistance protein ArsH -
HUZ74_RS17295 (3563478) 3563478..3565397 - 1920 Protein_3398 Tn3 family transposase -
HUZ74_RS17300 (3565497) 3565497..3566201 - 705 WP_001067858.1 IS6-like element IS26 family transposase -
HUZ74_RS17305 (3566260) 3566260..3566706 + 447 WP_239594482.1 hypothetical protein -
HUZ74_RS17310 (3566868) 3566868..3567284 - 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
HUZ74_RS17315 (3567281) 3567281..3567511 - 231 WP_001261287.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
HUZ74_RS17320 (3567807) 3567807..3568079 + 273 WP_061602529.1 hypothetical protein -
HUZ74_RS17325 (3568126) 3568126..3568830 + 705 WP_001067858.1 IS6-like element IS26 family transposase -
HUZ74_RS17330 (3568883) 3568883..3569476 + 594 Protein_3405 class A beta-lactamase -
HUZ74_RS17335 (3569509) 3569509..3569943 - 435 WP_000429836.1 Hg(II)-responsive transcriptional regulator -
HUZ74_RS17340 (3570015) 3570015..3570365 + 351 WP_001294663.1 mercuric transport protein MerT -
HUZ74_RS17345 (3570379) 3570379..3570654 + 276 WP_000732292.1 mercury resistance system periplasmic binding protein MerP -
HUZ74_RS17350 (3570690) 3570690..3571112 + 423 WP_001340589.1 organomercurial transporter MerC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island aph(3')-Ia / sul3 / ant(3'')-Ia / cmlA1 / aadA2 / dfrA12 / tet(A) - 3524266..3596514 72248
- inside IScluster/Tn - - 3563478..3568830 5352


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T161111 WP_001044768.1 NZ_CP055030:c3567284-3566868 [Shigella flexneri]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

>T161111 NZ_CP071027:c5282901-5282518 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT161111 WP_001261287.1 NZ_CP055030:c3567511-3567281 [Shigella flexneri]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT161111 NZ_CP071027:c5283143-5282901 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References