Detailed information of TA system TA161109

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2449996..2450634
• Replicon: chromosome
• Accession: NZ_CP055030
• Organism: Shigella flexneri strain SWHEFF_64

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: HUZ74_RS11845
• Protein ID: WP_000813794.1
• Coordinates: 2450458..2450634 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: HUZ74_RS11840
• Protein ID: WP_105966292.1
• Coordinates: 2449996..2450412 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ74_RS11820 (2445148)	2445148..2446089	-	942	WP_001251319.1	ABC transporter permease	-
HUZ74_RS11825 (2446090)	2446090..2447103	-	1014	WP_000220393.1	ABC transporter ATP-binding protein	-
HUZ74_RS11830 (2447121)	2447121..2448266	-	1146	WP_124846286.1	ABC transporter substrate-binding protein	-
HUZ74_RS11835 (2448511)	2448511..2449917	-	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
HUZ74_RS11840 (2449996)	2449996..2450412	-	417	WP_105966292.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
HUZ74_RS11845 (2450458)	2450458..2450634	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
HUZ74_RS11850 (2450856)	2450856..2451086	+	231	WP_000494244.1	YncJ family protein	-
HUZ74_RS11855 (2451178)	2451178..2453139	-	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
HUZ74_RS11860 (2453212)	2453212..2453748	-	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
HUZ74_RS11865 (2453840)	2453840..2455015	+	1176	WP_032253412.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-67)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T161109 WP_000813794.1 NZ_CP055030:c2450634-2450458 [Shigella flexneri]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T161109 NZ_CP071027:c4798768-4798484 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCAGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15228.60 Da
• Isoelectric Point: 4.7386

>AT161109 WP_105966292.1 NZ_CP055030:c2450412-2449996 [Shigella flexneri]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVIV

Nucleotide

• Length: 417 bp

>AT161109 NZ_CP071027:c4799000-4798758 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCAGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Toxin

• PDB: 6HPB