Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 840962..841616 Replicon chromosome
Accession NZ_CP055030
Organism Shigella flexneri strain SWHEFF_64

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag HUZ74_RS04090 Protein ID WP_000244777.1
Coordinates 841209..841616 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag HUZ74_RS04085 Protein ID WP_000354046.1
Coordinates 840962..841228 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ74_RS04060 (836131) 836131..836874 + 744 WP_000951962.1 SDR family oxidoreductase -
HUZ74_RS04065 (836931) 836931..838364 - 1434 WP_001338826.1 6-phospho-beta-glucosidase BglA -
HUZ74_RS04070 (838409) 838409..838720 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
HUZ74_RS04075 (838884) 838884..839543 + 660 WP_000250274.1 hemolysin III family protein -
HUZ74_RS04080 (839739) 839739..840719 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
HUZ74_RS04085 (840962) 840962..841228 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
HUZ74_RS04090 (841209) 841209..841616 + 408 WP_000244777.1 protein YgfX Toxin
HUZ74_RS04095 (841656) 841656..842177 - 522 WP_001055874.1 flavodoxin FldB -
HUZ74_RS04100 (842289) 842289..843185 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
HUZ74_RS04105 (843210) 843210..843920 + 711 WP_124846177.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
HUZ74_RS04110 (843926) 843926..845659 + 1734 WP_000813201.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T161096 WP_000244777.1 NZ_CP055030:841209-841616 [Shigella flexneri]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T161096 NZ_CP071025:c3717416-3717006 [Enterobacter hormaechei]
GTGGTCCTGTGGCAATCTGATCTTCGCGTCTCGTGGCGCTCACAGTGGATGTCCTTACTGCTCCACGGTCTGGTCGCGGC
AATGGTGTTATTGGTGCCGTGGCCGCTAAGCTATACCCCTCTGTGGCTGCTTTTGCTGTCGTTTGTAGTGTTTGACAGCG
TGCGCAGCCAGCGTCGAATTAACGCCCGTCAGGGTGAAATTAAGCTGCTGATGGATTCTCGCCTGCGCTGGCAGGGAAAA
GAGTGGGAAATGATAGGGACGCCGTGGATGCTCAACACCGGGATGATGCTACGGCTGCGTCGGGTCGAGGACAATCGTCG
CCAGCACCTGTGGCTGGCAGCAGACAGTATGGATCCTGCGGAGTGGCGAGATCTGCGCCGGTTGATCGTGCAACAGCCGA
CGCAGGAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT161096 WP_000354046.1 NZ_CP055030:840962-841228 [Shigella flexneri]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT161096 NZ_CP071025:c3717663-3717397 [Enterobacter hormaechei]
ATGGATATTAACAACAAGGCCCGTATTCACTGGGCGTGCCGTCGCGGCATGCGTGAACTTGATATCTCCATCATGCCTTT
CTTCGAGTATGAGTACGACAGCTTAAGCGATGACGATAAGCGCCTGTTTGTCCGTCTGCTTGAGTCCGACGATCCGGATT
TATTCAACTGGCTGATGAATCACGGCAAGCCCGCCGACACCGAGTTGCAACGGATGGTGCAATTAATTCAAACACGGAAT
CAGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References