Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-DnaT
Location 9177..9699 Replicon plasmid unnamed2
Accession NZ_CP055026
Organism Shigella flexneri strain SWHEFF_67

Toxin (Protein)


Gene name relE Uniprot ID G3CAN5
Locus tag HUZ75_RS22800 Protein ID WP_000220560.1
Coordinates 9418..9699 (+) Length 94 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID G3CAG1
Locus tag HUZ75_RS22795 Protein ID WP_000121743.1
Coordinates 9177..9428 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ75_RS22760 (HUZ75_22605) 4540..4770 + 231 WP_000051064.1 plasmid partition protein ParG -
HUZ75_RS22765 (HUZ75_22610) 4786..5079 + 294 WP_015060510.1 hypothetical protein -
HUZ75_RS22770 (HUZ75_22615) 5081..6049 + 969 WP_077249520.1 IS5-like element IS903B family transposase -
HUZ75_RS22775 (HUZ75_22620) 6109..6462 - 354 WP_000876434.1 DNA distortion polypeptide 3 -
HUZ75_RS22780 (HUZ75_22630) 6599..7045 - 447 WP_001074386.1 hypothetical protein -
HUZ75_RS22785 (HUZ75_22635) 7085..7921 - 837 WP_001050931.1 RepB family plasmid replication initiator protein -
HUZ75_RS22790 8173..8391 + 219 WP_128853136.1 hypothetical protein -
HUZ75_RS22795 (HUZ75_22640) 9177..9428 + 252 WP_000121743.1 plasmid stabilization protein Antitoxin
HUZ75_RS22800 (HUZ75_22645) 9418..9699 + 282 WP_000220560.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HUZ75_RS22805 (HUZ75_22650) 9845..10168 + 324 WP_001181903.1 hypothetical protein -
HUZ75_RS22810 (HUZ75_22655) 10213..10458 + 246 WP_000356546.1 hypothetical protein -
HUZ75_RS22815 (HUZ75_22660) 10448..10663 + 216 WP_001180116.1 hypothetical protein -
HUZ75_RS22820 (HUZ75_22665) 10756..11085 + 330 WP_000866648.1 hypothetical protein -
HUZ75_RS22825 (HUZ75_22670) 11372..11521 + 150 WP_000003880.1 hypothetical protein -
HUZ75_RS22830 (HUZ75_22675) 11534..11806 + 273 WP_000160399.1 hypothetical protein -
HUZ75_RS22835 (HUZ75_22680) 12133..12678 + 546 WP_038976855.1 DNA distortion polypeptide 1 -
HUZ75_RS22840 (HUZ75_22685) 12681..13838 + 1158 WP_000538023.1 DNA distortion polypeptide 2 -
HUZ75_RS22845 (HUZ75_22690) 14199..14690 + 492 WP_000872475.1 transcription termination/antitermination NusG family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaTEM-176 / aph(3')-Ia / sul3 / ant(3'')-Ia / floR - 1..47888 47888


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 11005.83 Da        Isoelectric Point: 10.5938

>T161092 WP_000220560.1 NZ_CP055026:9418-9699 [Shigella flexneri]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADLR

Download         Length: 282 bp

>T161092 NZ_CP071025:c1080523-1080305 [Enterobacter hormaechei]
ATGTCTGATAAACCATTAACTAAAGTCGATTATTTGATGCGCCTGCGACGCTGTCAGTCAATTGACACGCTCGAACGAGT
TATTGAGAAAAATAAATACGAGCTGTCTGATAATGAACTGGCGGTATTTTATTCAGCTGCCGACCATCGTCTTGCTGAAC
TGACCATGAATAAACTCTATGACAAGATCCCCTCTTCGGTATGGAAATTTGTTCGTTAG

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9194.43 Da        Isoelectric Point: 4.1683

>AT161092 WP_000121743.1 NZ_CP055026:9177-9428 [Shigella flexneri]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Download         Length: 252 bp

>AT161092 NZ_CP071025:c1080926-1080552 [Enterobacter hormaechei]
TTGGACGAGTACTCGCCAAAAAGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAATCTTGAAGAAAGTAACCATGGCTGGGTGAACGATCCAACGTCTGCTATCAATTTACAGCTTAATGAGCTGATCGAGC
ATATCGCAACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAGTTGATAGAGCAAATTGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTACGGCATTAACGCACAGGATTTGCAGAAATGGCGTAAATCAGGAAACCGTCT
ATTTCGCTGCTTCACGAATGTCAGCAGAGCTAATCCGGTTAGTCTTTCCTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G3CAN5

Antitoxin

Source ID Structure
AlphaFold DB A0A3S5I301

References