Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 94557..95158 Replicon plasmid unnamed1
Accession NZ_CP055025
Organism Shigella flexneri strain SWHEFF_67

Toxin (Protein)


Gene name doc Uniprot ID F0JYA5
Locus tag HUZ75_RS22700 Protein ID WP_001216044.1
Coordinates 94778..95158 (+) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag HUZ75_RS22695 Protein ID WP_001190712.1
Coordinates 94557..94778 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ75_RS22650 (HUZ75_22500) 89803..89982 + 180 Protein_102 hypothetical protein -
HUZ75_RS22655 (HUZ75_22505) 90332..90625 + 294 WP_039022009.1 hypothetical protein -
HUZ75_RS22660 (HUZ75_22510) 90625..90789 + 165 WP_152950163.1 hypothetical protein -
HUZ75_RS22665 (HUZ75_22515) 90856..91230 + 375 WP_039022008.1 hypothetical protein -
HUZ75_RS22670 (HUZ75_22520) 91212..91961 + 750 WP_171841598.1 hypothetical protein -
HUZ75_RS22675 (HUZ75_22525) 92031..92297 + 267 WP_223671585.1 hypothetical protein -
HUZ75_RS22680 (HUZ75_22530) 93087..93338 + 252 WP_039022007.1 DNA polymerase III subunit theta -
HUZ75_RS22685 (HUZ75_22535) 93345..93920 - 576 WP_039022006.1 hypothetical protein -
HUZ75_RS22690 (HUZ75_22540) 94095..94484 + 390 WP_000506730.1 S24 family peptidase -
HUZ75_RS22695 (HUZ75_22545) 94557..94778 + 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
HUZ75_RS22700 (HUZ75_22550) 94778..95158 + 381 WP_001216044.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
HUZ75_RS22705 (HUZ75_22555) 95163..95342 + 180 WP_039022005.1 hypothetical protein -
HUZ75_RS22710 (HUZ75_22560) 95370..96239 + 870 Protein_114 DUF968 domain-containing protein -
HUZ75_RS22715 96247..96381 + 135 WP_215243272.1 phage antirepressor KilAC domain-containing protein -
HUZ75_RS22720 (HUZ75_22565) 96414..97265 - 852 WP_000611653.1 phage repressor protein C1 -
HUZ75_RS22725 (HUZ75_22570) 97376..97585 - 210 WP_000874154.1 hypothetical protein -
HUZ75_RS22150 (HUZ75_21985) 97776..98457 + 682 WP_223678491.1 IS1-like element IS1B family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid tet(A) / qnrS1 / dfrA14 - 1..98457 98457
- flank IS/Tn - - 97776..98051 275


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(2-123)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13544.28 Da        Isoelectric Point: 5.6343

>T161091 WP_001216044.1 NZ_CP055025:94778-95158 [Shigella flexneri]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVAATLRRLYGSAE

Download         Length: 381 bp

>T161091 NZ_CP071025:30480-30794 [Enterobacter hormaechei]
ATGCATTTAATCTCAATGAAGGCAATTTTGGATGCGGTAAGCCAGTTCCCGCAATACAGAGAAGAGCTTCTCTTTTTAGG
ACGAGTTATTGAAAAGAGCCACTGCCCGACGCCCGCTGCACTGAGAAAACTTTTCCCAACGCTGGATAACTTCAAGTATC
TTGATAAGCATTATGTTATTGATATTGCAAATAACAATCTCAGAGTAGTGGCTCTCATATTCTTTGAAAGCCAAAAGTTT
TATGTGCGCCATGTTTTTACTCATAAGGAATATGACCGTTTTACGGAGAAACATCGCACTAAGGGGAAGAAATGA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT161091 WP_001190712.1 NZ_CP055025:94557-94778 [Shigella flexneri]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT161091 NZ_CP071025:30791-31210 [Enterobacter hormaechei]
ATGATGATCGTCGCTGATGCAATGAAAGCTACGCATGCCCTTGTTGCGGCCGTTCCTTTACTGGGTGAACAGCCGAGCGA
GAAAGATTATAAGGATGCGCTTGAGCTGGTTGAGTATCTCCTTATGAACGAGCCCAATAGCCCCTTGCTGGATATTGTTT
GCGCCAGAATTAGCCATTACGAAGCTAATCGGCCAGAGATAGTCGCATTACGCAAGGAGATGGAGTCCGTTCCCGTCGGA
ATTGCAGTTTTGAGAACCTTGATGGATCAATACAATCTGACGATATCGGATTTTAAGGATGAAATTGGTAGTAAATCGAT
GGTTTCACGGGTTCTGAATGGTCAGAGACAGCTTACTCTGAACCATATTAAAAAGCTGGCGGCCAGATTTGGGGTATCAC
CTGCGCTATTTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6Y6HMN5

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References