Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4105380..4105877 Replicon chromosome
Accession NZ_CP055024
Organism Shigella flexneri strain SWHEFF_67

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag HUZ75_RS20025 Protein ID WP_001173343.1
Coordinates 4105380..4105553 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag HUZ75_RS20030 Protein ID WP_000398619.1
Coordinates 4105581..4105877 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ75_RS20015 (4102050) 4102050..4103507 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
HUZ75_RS20020 (4103744) 4103744..4105261 + 1518 WP_001295074.1 lysine--tRNA ligase -
HUZ75_RS20025 (4105380) 4105380..4105553 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
HUZ75_RS20030 (4105581) 4105581..4105877 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
HUZ75_RS20035 (4106104) 4106104..4106376 - 273 WP_000405651.1 GNAT family N-acetyltransferase -
HUZ75_RS20040 (4106388) 4106388..4106618 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
HUZ75_RS20045 (4106799) 4106799..4108430 + 1632 WP_001216477.1 sensor histidine kinase -
HUZ75_RS20050 (4108427) 4108427..4109146 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T161089 WP_001173343.1 NZ_CP055024:c4105553-4105380 [Shigella flexneri]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T161089 NZ_CP071023:c4623040-4622729 [Enterobacter hormaechei]
ATGCTATTCATTGAAACGGATATTTTTACAGAAGACGTCAAAACGTTACTGGATGACGACGAATATCATAGGTTCCAAAT
ATTCCTGGCAACGCAACCGGATTACGGCGATCTGATCCAGAATACGGGAGGCTTGCGAAAGATTCGCTGGCTTGCTGGCG
GTAAGGGAAAGCGTAGTGGAGTCAGAGTTATCTATTTTCACCGTACGCGTGAGTGTGAAATCAGGTTGCTGCTCATCTAT
CGCAAAGGGATCAAAGATGACCTCTCCGCGGGTGAAAAAGCCATACTGAAAAAGATGATTGAGAGGTGGTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT161089 WP_000398619.1 NZ_CP055024:c4105877-4105581 [Shigella flexneri]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT161089 NZ_CP071023:c4622728-4622438 [Enterobacter hormaechei]
ATGGATAAGGCGCTGTTTGAGCGATTAACCCAAAGCATTGCGCAAATCAACGAAATTGCCGAGGGCCAACGAGAGCCGTC
GCGTACGTTTCAAATTGATGCGATGAAAATAAAAGAAATTCGTCAGGCGTCCGGGTTGTCGCAGAGTAAATTCGCGAACT
TAATCTCAGTTAGCGTTGATACGTTGCGTAACTGGGAACAAGGCAGACGCTCACCTACAGGCCCCGCTAAAGCGCTATTG
CGCGCCATTGCTAACGACCCTCAACATGTCATCCCAGCCCTAACGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References