Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3425458..3426076 Replicon chromosome
Accession NZ_CP055024
Organism Shigella flexneri strain SWHEFF_67

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag HUZ75_RS16685 Protein ID WP_001291435.1
Coordinates 3425858..3426076 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag HUZ75_RS16680 Protein ID WP_000344800.1
Coordinates 3425458..3425832 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ75_RS16670 (3420547) 3420547..3421740 + 1194 WP_072711618.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
HUZ75_RS16675 (3421763) 3421763..3424912 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
HUZ75_RS16680 (3425458) 3425458..3425832 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
HUZ75_RS16685 (3425858) 3425858..3426076 + 219 WP_001291435.1 HHA domain-containing protein Toxin
HUZ75_RS16690 (3426248) 3426248..3426799 + 552 WP_000102564.1 maltose O-acetyltransferase -
HUZ75_RS16695 (3426915) 3426915..3427385 + 471 WP_000136192.1 YlaC family protein -
HUZ75_RS16700 (3427549) 3427549..3429098 + 1550 Protein_3282 cyclic-guanylate-specific phosphodiesterase PdeB -
HUZ75_RS16705 (3429140) 3429140..3429493 - 354 WP_000878140.1 DUF1428 domain-containing protein -
HUZ75_RS16715 (3429872) 3429872..3430183 + 312 WP_000409911.1 MGMT family protein -
HUZ75_RS16720 (3430214) 3430214..3430786 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T161086 WP_001291435.1 NZ_CP055024:3425858-3426076 [Shigella flexneri]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T161086 NZ_CP071023:3454399-3454779 [Enterobacter hormaechei]
ATGCAAACTTTACCGTTACCCGAACCTCGGGCGGCTGAGCCATGTCCGTCGCCCGTGCAGGTCTGGCAACTCCTGCTCAC
GCATCTGCTGGATAAGCATTATGGTCTCACACTCAATGACACGCCGTTTGGCGACGACGGCGTAATTCAGGCGCACATCA
ACGCCGGTATCTCTCTGTGCGACGCCGTCAACTTCATTGTGGAAAGGTATGAACTGTTGCGCACTGACCACAGCAGCTCC
AGCCTCACCGAGCACTCTTCACTGATTGGTGCCATCGACATCCTGCGTGCCCACCGTGCAACCGGCCTGATAGTGGGCAG
CGGCTACAGCACCATCACCCGCATCACAACGGGCCAGTATCGGGAGGACAAAACACAGTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT161086 WP_000344800.1 NZ_CP055024:3425458-3425832 [Shigella flexneri]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT161086 NZ_CP071023:3454040-3454366 [Enterobacter hormaechei]
ATGCATTCTGAACAATGGGGACTGACGTGCGGTAAAACGCCGTGCTTCAGTGCCAAGCTGGTGCAGGAAGGCAACCGGCT
GCACTTTCTAGCTGACCGAGCAGAGTTTAGTGGCGACTTGAGCGACGATGAGGCACTGCGCCTCGACCAGGCTTTTCCGG
TGATATTGAAGCAGCTGGAACAAATGCTTTCAGCCGGTGAGCTGAATCCCCGCCATCAGCATCGCGTCACTCTGCATCTT
AACGGGCTAACCTGCGAAGCCGACACGCTCGGTTCGCACGGCTACCTTTACATCGCGATTTATCCCTCAGCGGTAGCTAC
CGGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References