Detailed information of TA system TA161070

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 4452376..4452971
• Replicon: chromosome
• Accession: NZ_CP055022
• Organism: Escherichia coli strain SWHEFF_68

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: V0SXT4
• Locus tag: HUZ76_RS21305
• Protein ID: WP_000239581.1
• Coordinates: 4452376..4452726 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: L4JJX7
• Locus tag: HUZ76_RS21310
• Protein ID: WP_001223213.1
• Coordinates: 4452720..4452971 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ76_RS21285 (4447830)	4447830..4448852	-	1023	WP_001298067.1	ABC transporter permease	-
HUZ76_RS21290 (4448866)	4448866..4450368	-	1503	WP_022646474.1	sugar ABC transporter ATP-binding protein	-
HUZ76_RS21295 (4450501)	4450501..4451457	-	957	WP_000265933.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
HUZ76_RS21300 (4451767)	4451767..4452297	+	531	WP_000055075.1	inorganic diphosphatase	-
HUZ76_RS21305 (4452376)	4452376..4452726	-	351	WP_000239581.1	endoribonuclease toxin ChpB	Toxin
HUZ76_RS21310 (4452720)	4452720..4452971	-	252	WP_001223213.1	type II toxin-antitoxin system antitoxin ChpS	Antitoxin
HUZ76_RS21315 (4453183)	4453183..4453524	-	342	WP_001219160.1	gamma-glutamylcyclotransferase	-
HUZ76_RS21320 (4453527)	4453527..4457306	-	3780	WP_022646473.1	autotransporter assembly complex protein TamB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12453.41 Da
• Isoelectric Point: 6.2206

>T161070 WP_000239581.1 NZ_CP055022:c4452726-4452376 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAAGEVVEEALLRLQAVVE

Nucleotide

• Length: 351 bp

>T161070 NZ_CP071019:c3827648-3827157 [Enterobacter hormaechei]
ATGATCTCTGCCCCAGTGCCCCTTCGAGCCGGGCATATTCTTACTCCATTTTGCTGTGGGGTAGATTCGATGGATAACTG
GCTTAAGCAGCGGGCGATGAAAAATCAGGCCAGTGGTGCATCGCGTACATTTGTCTGTTGTGGCGGAGATTCTAACGTAC
TGGCTTATTACGCGCTGGCGTCCAGCGCGGTCACAACGAATACGGTACCCGGCCGCTTTCGTCGCAATATGCCCGATCCC
ATCCCTGTTGTAGTGCTGGGACGCCTGGCGGTAGATCAGTCTCTTCATGGGCAGGGCGTTGGTCGGGCGCTGGTACGCGA
CGCCGGGCTGCGGGTGATCCAGGTGGCGGAGAGCATCGGCATCCGCGGAATGCTGGTTCATGCCCTGTCGGATGAAGCGC
GGGAATTTTATCTGCGGGTGGGGTTTGAGCCATCGCCGATGGATCCGATGATATTGATGGTGACGTTGGGGGATTTGGTG
GCGAGTATTTAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9331.64 Da
• Isoelectric Point: 4.6509

>AT161070 WP_001223213.1 NZ_CP055022:c4452971-4452720 [Escherichia coli]
MRITIKRWGNSSGMVIPNVVMKELNLRPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNATELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT161070 NZ_CP071019:c3827938-3827645 [Enterobacter hormaechei]
ATGCCAGCTGCAAACACTATGGCGGTGAAACGCGAAACCCTTAACCTGCGCATCAGGCCTGCCGAGCGGGATCTTATCGA
TCGGGCAGCGAAAGCGAGAGGGAAAAACCGTACTGACTTTGTGCTGGAAGCAGCCCGCGCAGCTGCGGAAGAGGCACTGA
TCGAACAACGCATCATAATGGCCGATCCAGAGGCTTATCAGGAATTTCTGACCCGTCTGGATCAGGCTCCAGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCTTGCGCCGTGGGAGCAGGAAAAATGA

Structures

Toxin

• AlphaFold DB: V0SXT4

Antitoxin

• AlphaFold DB: L4JJX7