Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 4222681..4223231 Replicon chromosome
Accession NZ_CP055022
Organism Escherichia coli strain SWHEFF_68

Toxin (Protein)


Gene name ccdB Uniprot ID S1PDG4
Locus tag HUZ76_RS20230 Protein ID WP_001160966.1
Coordinates 4222681..4222995 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1NMQ0
Locus tag HUZ76_RS20235 Protein ID WP_000125568.1
Coordinates 4222998..4223231 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ76_RS20205 (4218356) 4218356..4219642 + 1287 WP_000800457.1 peptidylprolyl isomerase SurA -
HUZ76_RS20210 (4219642) 4219642..4220631 + 990 WP_000241224.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
HUZ76_RS20215 (4220628) 4220628..4221449 + 822 WP_001065381.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
HUZ76_RS20220 (4221452) 4221452..4221829 + 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
HUZ76_RS20225 (4221836) 4221836..4222684 + 849 WP_000257195.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
HUZ76_RS20230 (4222681) 4222681..4222995 - 315 WP_001160966.1 CcdB family protein Toxin
HUZ76_RS20235 (4222998) 4222998..4223231 - 234 WP_000125568.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
HUZ76_RS20240 (4223317) 4223317..4223796 - 480 WP_000624375.1 type 3 dihydrofolate reductase -
HUZ76_RS20245 (4223988) 4223988..4225850 - 1863 WP_022645165.1 glutathione-regulated potassium-efflux system protein KefC -
HUZ76_RS20250 (4225843) 4225843..4226373 - 531 WP_000600732.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
HUZ76_RS20255 (4226481) 4226481..4227812 - 1332 WP_001183201.1 MFS transporter -
HUZ76_RS20260 (4227870) 4227870..4228157 - 288 WP_000203741.1 ferredoxin-like protein FixX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.72 Da        Isoelectric Point: 9.0851

>T161069 WP_001160966.1 NZ_CP055022:c4222995-4222681 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T161069 NZ_CP071019:c3734628-3734218 [Enterobacter hormaechei]
GTGGTCCTGTGGCAATCTGATCTTCGCGTCTCGTGGCGCTCACAGTGGATGTCCTTACTGCTCCACGGTCTGGTCGCGGC
AATGGTGTTATTGGTGCCGTGGCCGCTAAGCTATACCCCTCTGTGGCTGCTTTTGCTGTCGTTTGTAGTGTTTGACAGCG
TGCGCAGCCAGCGTCGAATTAACGCCCGTCAGGGTGAAATTAAGCTGCTGATGGATTCTCGCCTGCGCTGGCAGGGAAAA
GAGTGGGAAATGATAGGGACGCCGTGGATGCTCAACACCGGGATGATGCTACGGCTGCGTCGGGTCGAGGACAATCGTCG
CCAGCACCTGTGGCTGGCAGCAGACAGTATGGATCCTGCGGAGTGGCGAGATCTGCGCCGGTTGATCGTGCAACAGCCGA
CGCAGGAGTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8560.55 Da        Isoelectric Point: 5.8062

>AT161069 WP_000125568.1 NZ_CP055022:c4223231-4222998 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENTEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT161069 NZ_CP071019:c3734875-3734609 [Enterobacter hormaechei]
ATGGATATTAACAACAAGGCCCGTATTCACTGGGCGTGCCGTCGCGGCATGCGTGAACTTGATATCTCCATCATGCCTTT
CTTCGAGTATGAGTACGACAGCTTAAGCGATGACGATAAGCGCCTGTTTGTCCGTCTGCTTGAGTCCGACGATCCGGATT
TATTCAACTGGCTGATGAATCACGGCAAGCCCGCCGACACCGAGTTGCAACGGATGGTGCAATTAATTCAAACACGGAAT
CAGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G815

Antitoxin

Source ID Structure
AlphaFold DB A0A829GEF2

References