Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3742931..3743549 Replicon chromosome
Accession NZ_CP055022
Organism Escherichia coli strain SWHEFF_68

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag HUZ76_RS17900 Protein ID WP_001291435.1
Coordinates 3743331..3743549 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag HUZ76_RS17895 Protein ID WP_000344800.1
Coordinates 3742931..3743305 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ76_RS17885 (3738020) 3738020..3739213 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
HUZ76_RS17890 (3739236) 3739236..3742385 + 3150 WP_112918615.1 efflux RND transporter permease AcrB -
HUZ76_RS17895 (3742931) 3742931..3743305 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
HUZ76_RS17900 (3743331) 3743331..3743549 + 219 WP_001291435.1 HHA domain-containing protein Toxin
HUZ76_RS17905 (3743721) 3743721..3744272 + 552 WP_000536391.1 maltose O-acetyltransferase -
HUZ76_RS17910 (3744388) 3744388..3744858 + 471 WP_022645280.1 YlaC family protein -
HUZ76_RS17915 (3745022) 3745022..3746572 + 1551 WP_012602175.1 cyclic-guanylate-specific phosphodiesterase PdeB -
HUZ76_RS17920 (3746613) 3746613..3746966 - 354 WP_000878140.1 DUF1428 domain-containing protein -
HUZ76_RS17930 (3747345) 3747345..3747656 + 312 WP_000409911.1 MGMT family protein -
HUZ76_RS17935 (3747687) 3747687..3748259 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T161067 WP_001291435.1 NZ_CP055022:3743331-3743549 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T161067 NZ_CP071019:c2708352-2708249 [Enterobacter hormaechei]
GGCAAGGCGATTGAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT161067 WP_000344800.1 NZ_CP055022:3742931-3743305 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT161067 NZ_CP071019:2708244-2708445 [Enterobacter hormaechei]
CAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTT
GGCATTAATGCAGGCTCAATCGCCTTGCCCTTTAAGAATAGATGACGACGTCAGGTTTTCCAGTCCACAGTAAAAGTGGT
CTGAAAAAAAGCGTCAGAACATCACTAAATGTGAAAAACCGC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References