Detailed information of TA system TA161065

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 3344113..3344818
• Replicon: chromosome
• Accession: NZ_CP055022
• Organism: Escherichia coli strain SWHEFF_68

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: HUZ76_RS16125
• Protein ID: WP_000539521.1
• Coordinates: 3344113..3344499 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: HUZ76_RS16130
• Protein ID: WP_001280945.1
• Coordinates: 3344489..3344818 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ76_RS16105 (3340117)	3340117..3340743	+	627	WP_001295292.1	glutathione S-transferase GstB	-
HUZ76_RS16110 (3340740)	3340740..3341855	-	1116	WP_000554964.1	aldose sugar dehydrogenase YliI	-
HUZ76_RS16115 (3341966)	3341966..3342349	-	384	WP_000497137.1	biofilm formation regulator BssR	-
HUZ76_RS16120 (3342562)	3342562..3343887	+	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
HUZ76_RS16125 (3344113)	3344113..3344499	+	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HUZ76_RS16130 (3344489)	3344489..3344818	+	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
HUZ76_RS16135 (3344888)	3344888..3346216	-	1329	WP_022645414.1	GGDEF domain-containing protein	-
HUZ76_RS16140 (3346224)	3346224..3348572	-	2349	WP_022645413.1	EAL domain-containing protein	-
HUZ76_RS16145 (3348749)	3348749..3349660	-	912	WP_001236044.1	glutathione ABC transporter permease GsiD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T161065 WP_000539521.1 NZ_CP055022:3344113-3344499 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T161065 NZ_CP071019:c1080528-1080310 [Enterobacter hormaechei]
ATGTCTGATAAACCATTAACTAAAGTCGATTATTTGATGCGCCTGCGACGCTGTCAGTCAATTGACACGCTCGAACGAGT
TATTGAGAAAAATAAATACGAGCTGTCTGATAATGAACTGGCGGTATTTTATTCAGCTGCCGACCATCGTCTTGCTGAAC
TGACCATGAATAAACTCTATGACAAGATCCCCTCTTCGGTATGGAAATTTGTTCGTTAG

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT161065 WP_001280945.1 NZ_CP055022:3344489-3344818 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT161065 NZ_CP071019:c1080931-1080557 [Enterobacter hormaechei]
TTGGACGAGTACTCGCCAAAAAGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAATCTTGAAGAAAGTAACCATGGCTGGGTGAACGATCCAACGTCTGCTATCAATTTACAGCTTAATGAGCTGATCGAGC
ATATCGCAACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAGTTGATAGAGCAAATTGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTACGGCATTAACGCACAGGATTTGCAGAAATGGCGTAAATCAGGAAACCGTCT
ATTTCGCTGCTTCACGAATGTCAGCAGAGCTAATCCGGTTAGTCTTTCCTGTTAA

Structures

Toxin

• AlphaFold DB: A0A829GCT6