Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 3016560..3017344 Replicon chromosome
Accession NZ_CP055022
Organism Escherichia coli strain SWHEFF_68

Toxin (Protein)


Gene name TacT2 Uniprot ID V0T0H9
Locus tag HUZ76_RS14585 Protein ID WP_000613626.1
Coordinates 3016850..3017344 (+) Length 165 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID A0A0A1AEM7
Locus tag HUZ76_RS14580 Protein ID WP_024946541.1
Coordinates 3016560..3016853 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ76_RS14570 (3011721) 3011721..3012680 - 960 WP_022645527.1 23S rRNA pseudouridine(955/2504/2580) synthase RluC -
HUZ76_RS14575 (3013253) 3013253..3016426 + 3174 WP_063814920.1 ribonuclease E -
HUZ76_RS14580 (3016560) 3016560..3016853 + 294 WP_024946541.1 DUF1778 domain-containing protein Antitoxin
HUZ76_RS14585 (3016850) 3016850..3017344 + 495 WP_000613626.1 GNAT family N-acetyltransferase Toxin
HUZ76_RS14590 (3017439) 3017439..3018392 - 954 WP_022645524.1 flagellar hook-associated protein FlgL -
HUZ76_RS14595 (3018404) 3018404..3020047 - 1644 WP_022645523.1 flagellar hook-associated protein FlgK -
HUZ76_RS14600 (3020113) 3020113..3021054 - 942 WP_001317765.1 flagellar assembly peptidoglycan hydrolase FlgJ -
HUZ76_RS14605 (3021054) 3021054..3022151 - 1098 WP_021580666.1 flagellar basal body P-ring protein FlgI -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(81-144)

Antitoxin

Vibrio phage ICP1, Orf50

(14-88)


Sequences

Toxin        


Download         Length: 165 a.a.        Molecular weight: 18084.00 Da        Isoelectric Point: 8.2617

>T161062 WP_000613626.1 NZ_CP055022:3016850-3017344 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Download         Length: 495 bp

>T161062 NZ_CP071017:c4655489-4655178 [Enterobacter hormaechei]
ATGCTATTCATTGAAACGGATATTTTTACAGAAGACGTCAAAACGTTACTGGATGACGACGAATATCATAGGTTCCAAAT
ATTCCTGGCAACGCAACCGGATTACGGCGATCTGATCCAGAATACGGGAGGCTTGCGAAAGATTCGCTGGCTTGCTGGCG
GTAAGGGAAAGCGTAGTGGAGTCAGAGTTATCTATTTTCACCGTACGCGTGAGTGTGAAATCAGGTTGCTGCTCATCTAT
CGCAAAGGGATCAAAGATGACCTCTCCGCGGGTGAAAAAGCCATACTGAAAAAGATGATTGAGAGGTGGTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11039.60 Da        Isoelectric Point: 10.5423

>AT161062 WP_024946541.1 NZ_CP055022:3016560-3016853 [Escherichia coli]
MAAANNIPARRESLNLRIKPAERDLIDRAAKARGKNRSEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Download         Length: 294 bp

>AT161062 NZ_CP071017:c4655177-4654887 [Enterobacter hormaechei]
ATGGATAAGGCGCTGTTTGAGCGATTAACCCAAAGCATTGCGCAAATCAACGAAATTGCCGAGGGCCAACGAGAGCCGTC
GCGTACGTTTCAAATTGATGCGATGAAAATAAAAGAAATTCGTCAGGCGTCCGGGTTGTCGCAGAGTAAATTCGCGAACT
TAATCTCAGTTAGCGTTGATACGTTGCGTAACTGGGAACAAGGCAGACGCTCACCTACAGGCCCCGCTAAAGCGCTATTG
CGCGCCATTGCTAACGACCCTCAACATGTCATCCCAGCCCTAACGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0T0H9

Antitoxin

Source ID Structure
AlphaFold DB A0A0A1AEM7

References