Detailed information of TA system TA161061

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2614734..2615372
• Replicon: chromosome
• Accession: NZ_CP055022
• Organism: Escherichia coli strain SWHEFF_68

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: HUZ76_RS12560
• Protein ID: WP_000813794.1
• Coordinates: 2615196..2615372 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: HUZ76_RS12555
• Protein ID: WP_001270286.1
• Coordinates: 2614734..2615150 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ76_RS12535 (2609886)	2609886..2610827	-	942	WP_088129997.1	ABC transporter permease	-
HUZ76_RS12540 (2610828)	2610828..2611841	-	1014	WP_022645666.1	ABC transporter ATP-binding protein	-
HUZ76_RS12545 (2611859)	2611859..2613004	-	1146	WP_000047466.1	ABC transporter substrate-binding protein	-
HUZ76_RS12550 (2613249)	2613249..2614655	-	1407	WP_022645665.1	PLP-dependent aminotransferase family protein	-
HUZ76_RS12555 (2614734)	2614734..2615150	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
HUZ76_RS12560 (2615196)	2615196..2615372	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
HUZ76_RS12565 (2615594)	2615594..2615824	+	231	WP_000491567.1	DUF2554 family protein	-
HUZ76_RS12570 (2615916)	2615916..2617877	-	1962	WP_023909195.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
HUZ76_RS12575 (2617950)	2617950..2618486	-	537	WP_000429148.1	DNA-binding transcriptional regulator SutR	-
HUZ76_RS12580 (2618578)	2618578..2619750	+	1173	WP_022645663.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T161061 WP_000813794.1 NZ_CP055022:c2615372-2615196 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T161061 NZ_CP071017:c3827648-3827157 [Enterobacter hormaechei]
ATGATCTCTGCCCCAGTGCCCCTTCGAGCCGGGCATATTCTTACTCCATTTTGCTGTGGGGTAGATTCGATGGATAACTG
GCTTAAGCAGCGGGCGATGAAAAATCAGGCCAGTGGTGCATCGCGTACATTTGTCTGTTGTGGCGGAGATTCTAACGTAC
TGGCTTATTACGCGCTGGCGTCCAGCGCGGTCACAACGAATACGGTACCCGGCCGCTTTCGTCGCAATATGCCCGATCCC
ATCCCTGTTGTAGTGCTGGGACGCCTGGCGGTAGATCAGTCTCTTCATGGGCAGGGCGTTGGTCGGGCGCTGGTACGCGA
CGCCGGGCTGCGGGTGATCCAGGTGGCGGAGAGCATCGGCATCCGCGGAATGCTGGTTCATGCCCTGTCGGATGAAGCGC
GGGAATTTTATCTGCGGGTGGGGTTTGAGCCATCGCCGATGGATCCGATGATATTGATGGTGACGTTGGGGGATTTGGTG
GCGAGTATTTAG

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT161061 WP_001270286.1 NZ_CP055022:c2615150-2614734 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT161061 NZ_CP071017:c3827938-3827645 [Enterobacter hormaechei]
ATGCCAGCTGCAAACACTATGGCGGTGAAACGCGAAACCCTTAACCTGCGCATCAGGCCTGCCGAGCGGGATCTTATCGA
TCGGGCAGCGAAAGCGAGAGGGAAAAACCGTACTGACTTTGTGCTGGAAGCAGCCCGCGCAGCTGCGGAAGAGGCACTGA
TCGAACAACGCATCATAATGGCCGATCCAGAGGCTTATCAGGAATTTCTGACCCGTCTGGATCAGGCTCCAGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCTTGCGCCGTGGGAGCAGGAAAAATGA

Structures

Toxin

• PDB: 6HPB