Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 166360..166972 Replicon chromosome
Accession NZ_CP055022
Organism Escherichia coli strain SWHEFF_68

Toxin (Protein)


Gene name hicA Uniprot ID U9YXE2
Locus tag HUZ76_RS00725 Protein ID WP_000833473.1
Coordinates 166360..166545 (+) Length 62 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A0A1AGA0
Locus tag HUZ76_RS00730 Protein ID WP_022646255.1
Coordinates 166562..166972 (+) Length 137 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ76_RS00710 (161826) 161826..163121 + 1296 WP_000985736.1 Fic family protein -
HUZ76_RS00715 (163229) 163229..164767 + 1539 WP_000183976.1 aldehyde dehydrogenase AldB -
HUZ76_RS00720 (164808) 164808..165887 - 1080 WP_000061477.1 type I restriction enzyme HsdR N-terminal domain-containing protein -
HUZ76_RS00725 (166360) 166360..166545 + 186 WP_000833473.1 type II toxin-antitoxin system HicA family toxin Toxin
HUZ76_RS00730 (166562) 166562..166972 + 411 WP_022646255.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
HUZ76_RS00735 (167093) 167093..169063 - 1971 WP_077517025.1 glycoside hydrolase family 127 protein -
HUZ76_RS00740 (169074) 169074..170474 - 1401 WP_000204814.1 MFS transporter -
HUZ76_RS00745 (170700) 170700..171515 + 816 WP_000891820.1 AraC family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(4-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-129)


Sequences

Toxin        


Download         Length: 62 a.a.        Molecular weight: 6800.89 Da        Isoelectric Point: 11.7053

>T161047 WP_000833473.1 NZ_CP055022:166360-166545 [Escherichia coli]
VKSADVIAILKQHGWEHIRTRGSHHQFRHPVHPGLVTVPHPKKDIKPGTLAQIGRQAGLTF

Download         Length: 186 bp

>T161047 NZ_CP071015:c1080529-1080311 [Enterobacter hormaechei]
ATGTCTGATAAACCATTAACTAAAGTCGATTATTTGATGCGCCTGCGACGCTGTCAGTCAATTGACACGCTCGAACGAGT
TATTGAGAAAAATAAATACGAGCTGTCTGATAATGAACTGGCGGTATTTTATTCAGCTGCCGACCATCGTCTTGCTGAAC
TGACCATGAATAAACTCTATGACAAGATCCCCTCTTCGGTATGGAAATTTGTTCGTTAG

Antitoxin


Download         Length: 137 a.a.        Molecular weight: 15214.08 Da        Isoelectric Point: 4.4482

>AT161047 WP_022646255.1 NZ_CP055022:166562-166972 [Escherichia coli]
MFYPAYIHSDPDGSASGFFPDVPGCYFAGDTLDNAFQDARSALAAHFETLCEMDEELPLPGSVETHLVQRAQDFIGGQWL
LVDINMNQFEGRAERINITMPKRLLNKIDTYVRNNPDYANRSAFLAEAARRVLPGV

Download         Length: 411 bp

>AT161047 NZ_CP071015:c1080932-1080558 [Enterobacter hormaechei]
TTGGACGAGTACTCGCCAAAAAGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAATCTTGAAGAAAGTAACCATGGCTGGGTGAACGATCCAACGTCTGCTATCAATTTACAGCTTAATGAGCTGATCGAGC
ATATCGCAACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAGTTGATAGAGCAAATTGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTACGGCATTAACGCACAGGATTTGCAGAAATGGCGTAAATCAGGAAACCGTCT
ATTTCGCTGCTTCACGAATGTCAGCAGAGCTAATCCGGTTAGTCTTTCCTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9YXE2

Antitoxin

Source ID Structure
AlphaFold DB A0A0A1AGA0

References