Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 132250..132893 Replicon plasmid unnamed1
Accession NZ_CP055020
Organism Escherichia coli strain SWHEFF_69

Toxin (Protein)


Gene name vagD Uniprot ID V0UN72
Locus tag HUZ77_RS24350 Protein ID WP_001034044.1
Coordinates 132477..132893 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID B1P7N7
Locus tag HUZ77_RS24345 Protein ID WP_001261286.1
Coordinates 132250..132480 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ77_RS24325 (128903) 128903..129658 - 756 WP_000852146.1 replication initiation protein RepE -
HUZ77_RS24330 (130358) 130358..131164 - 807 WP_000016970.1 site-specific integrase -
HUZ77_RS24335 (131165) 131165..131470 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB -
HUZ77_RS24340 (131472) 131472..131690 - 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA -
HUZ77_RS24345 (132250) 132250..132480 + 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
HUZ77_RS24350 (132477) 132477..132893 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin Toxin
HUZ77_RS24355 (132968) 132968..134533 + 1566 WP_001128474.1 AAA family ATPase -
HUZ77_RS24360 (134518) 134518..135540 + 1023 WP_000361402.1 helicase UvrD -
HUZ77_RS24365 (135794) 135794..136480 - 687 Protein_172 IS1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(M) / ant(3'')-Ia / cmlA1 / aadA2 / dfrA12 / floR / sul2 / tet(A) / lnu(F) / aac(3)-IId / aph(3')-Ia / sul3 / blaTEM-1B / sitABCD iutA / iucD / iucC / iucB / iucA 1..154739 154739
- flank IS/Tn - - 135794..136141 347


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15232.66 Da        Isoelectric Point: 6.8536

>T161046 WP_001034044.1 NZ_CP055020:132477-132893 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR

Download         Length: 417 bp

>T161046 NZ_CP071015:30480-30794 [Enterobacter hormaechei]
ATGCATTTAATCTCAATGAAGGCAATTTTGGATGCGGTAAGCCAGTTCCCGCAATACAGAGAAGAGCTTCTCTTTTTAGG
ACGAGTTATTGAAAAGAGCCACTGCCCGACGCCCGCTGCACTGAGAAAACTTTTCCCAACGCTGGATAACTTCAAGTATC
TTGATAAGCATTATGTTATTGATATTGCAAATAACAATCTCAGAGTAGTGGCTCTCATATTCTTTGAAAGCCAAAAGTTT
TATGTGCGCCATGTTTTTACTCATAAGGAATATGACCGTTTTACGGAGAAACATCGCACTAAGGGGAAGAAATGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8747.86 Da        Isoelectric Point: 4.3775

>AT161046 WP_001261286.1 NZ_CP055020:132250-132480 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMTEREDVVSDEGRVNL

Download         Length: 231 bp

>AT161046 NZ_CP071015:30791-31210 [Enterobacter hormaechei]
ATGATGATCGTCGCTGATGCAATGAAAGCTACGCATGCCCTTGTTGCGGCCGTTCCTTTACTGGGTGAACAGCCGAGCGA
GAAAGATTATAAGGATGCGCTTGAGCTGGTTGAGTATCTCCTTATGAACGAGCCCAATAGCCCCTTGCTGGATATTGTTT
GCGCCAGAATTAGCCATTACGAAGCTAATCGGCCAGAGATAGTCGCATTACGCAAGGAGATGGAGTCCGTTCCCGTCGGA
ATTGCAGTTTTGAGAACCTTGATGGATCAATACAATCTGACGATATCGGATTTTAAGGATGAAATTGGTAGTAAATCGAT
GGTTTCACGGGTTCTGAATGGTCAGAGACAGCTTACTCTGAACCATATTAAAAAGCTGGCGGCCAGATTTGGGGTATCAC
CTGCGCTATTTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CHW1

Antitoxin

Source ID Structure
AlphaFold DB A0A829CKZ6

References