Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpSB/PRK09812-ChpS
Location 4295447..4296042 Replicon chromosome
Accession NZ_CP055019
Organism Escherichia coli strain SWHEFF_69

Toxin (Protein)


Gene name chpB Uniprot ID V0SXT4
Locus tag HUZ77_RS20515 Protein ID WP_000239581.1
Coordinates 4295447..4295797 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name chpS Uniprot ID L4JJX7
Locus tag HUZ77_RS20520 Protein ID WP_001223213.1
Coordinates 4295791..4296042 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ77_RS20495 (4290901) 4290901..4291923 - 1023 WP_001361374.1 ABC transporter permease -
HUZ77_RS20500 (4291937) 4291937..4293439 - 1503 WP_001095661.1 ATP-binding cassette domain-containing protein -
HUZ77_RS20505 (4293572) 4293572..4294528 - 957 WP_108997067.1 galactofuranose ABC transporter substrate-binding protein YtfQ -
HUZ77_RS20510 (4294838) 4294838..4295368 + 531 WP_000055072.1 inorganic diphosphatase -
HUZ77_RS20515 (4295447) 4295447..4295797 - 351 WP_000239581.1 endoribonuclease toxin ChpB Toxin
HUZ77_RS20520 (4295791) 4295791..4296042 - 252 WP_001223213.1 type II toxin-antitoxin system antitoxin ChpS Antitoxin
HUZ77_RS20525 (4296254) 4296254..4296595 - 342 WP_001219160.1 gamma-glutamylcyclotransferase -
HUZ77_RS20530 (4296598) 4296598..4300377 - 3780 WP_108997070.1 autotransporter assembly complex protein TamB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-114)

Antitoxin

SpoVT-AbrB domain

(6-49)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12453.41 Da        Isoelectric Point: 6.2206

>T161035 WP_000239581.1 NZ_CP055019:c4295797-4295447 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAAGEVVEEALLRLQAVVE

Download         Length: 351 bp

>T161035 NZ_CP071011:c4624399-4624088 [Enterobacter hormaechei]
ATGCTATTCATTGAAACGGATATTTTTACAGAAGACGTCAAAACGTTACTGGATGACGACGAATATCATAGGTTCCAAAT
ATTCCTGGCAACGCAACCGGATTACGGCGATCTGATCCAGAATACGGGAGGCTTGCGAAAGATTCGCTGGCTTGCTGGCG
GTAAGGGAAAGCGTAGTGGAGTCAGAGTTATCTATTTTCACCGTACGCGTGAGTGTGAAATCAGGTTGCTGCTCATCTAT
CGCAAAGGGATCAAAGATGACCTCTCCGCGGGTGAAAAAGCCATACTGAAAAAGATGATTGAGAGGTGGTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9331.64 Da        Isoelectric Point: 4.6509

>AT161035 WP_001223213.1 NZ_CP055019:c4296042-4295791 [Escherichia coli]
MRITIKRWGNSSGMVIPNVVMKELNLRPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNATELSEQDVWGKSTPAGD
EIW

Download         Length: 252 bp

>AT161035 NZ_CP071011:c4624087-4623797 [Enterobacter hormaechei]
ATGGATAAGGCGCTGTTTGAGCGATTAACCCAAAGCATTGCGCAAATCAACGAAATTGCCGAGGGCCAACGAGAGCCGTC
GCGTACGTTTCAAATTGATGCGATGAAAATAAAAGAAATTCGTCAGGCGTCCGGGTTGTCGCAGAGTAAATTCGCGAACT
TAATCTCAGTTAGCGTTGATACGTTGCGTAACTGGGAACAAGGCAGACGCTCACCTACAGGCCCCGCTAAAGCGCTATTG
CGCGCCATTGCTAACGACCCTCAACATGTCATCCCAGCCCTAACGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SXT4

Antitoxin

Source ID Structure
AlphaFold DB L4JJX7

References