Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4189235..4189811 Replicon chromosome
Accession NZ_CP055019
Organism Escherichia coli strain SWHEFF_69

Toxin (Protein)


Gene name shpA Uniprot ID A0A2U2VE01
Locus tag HUZ77_RS20035 Protein ID WP_001131965.1
Coordinates 4189524..4189811 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A2U2VE39
Locus tag HUZ77_RS20030 Protein ID WP_000063158.1
Coordinates 4189235..4189537 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ77_RS20010 (4184343) 4184343..4185563 + 1221 WP_000873756.1 amino acid deaminase -
HUZ77_RS20015 (4185831) 4185831..4187981 + 2151 WP_001314407.1 pyruvate/proton symporter BtsT -
HUZ77_RS20020 (4188031) 4188031..4188234 + 204 WP_000467859.1 YbdD/YjiX family protein -
HUZ77_RS20025 (4188245) 4188245..4189201 + 957 WP_001361361.1 GTPase -
HUZ77_RS20030 (4189235) 4189235..4189537 - 303 WP_000063158.1 BrnA antitoxin family protein Antitoxin
HUZ77_RS20035 (4189524) 4189524..4189811 - 288 WP_001131965.1 BrnT family toxin Toxin
HUZ77_RS20040 (4190008) 4190008..4191174 + 1167 WP_000800823.1 restriction endonuclease -
HUZ77_RS20045 (4191219) 4191219..4191392 + 174 Protein_3936 type I restriction-modification system subunit M N-terminal domain-containing protein -
HUZ77_RS20050 (4191386) 4191386..4192054 + 669 Protein_3937 DUF3387 domain-containing protein -
HUZ77_RS20055 (4192269) 4192269..4192607 + 339 WP_000132633.1 endoribonuclease SymE -
HUZ77_RS20060 (4192695) 4192695..4192742 - 48 WP_229017048.1 hypothetical protein -
HUZ77_RS20065 (4192990) 4192990..4194402 + 1413 WP_000199309.1 PLP-dependent aminotransferase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11223.71 Da        Isoelectric Point: 7.4021

>T161033 WP_001131965.1 NZ_CP055019:c4189811-4189524 [Escherichia coli]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T161033 NZ_CP071011:c3734629-3734219 [Enterobacter hormaechei]
GTGGTCCTGTGGCAATCTGATCTTCGCGTCTCGTGGCGCTCACAGTGGATGTCCTTACTGCTCCACGGTCTGGTCGCGGC
AATGGTGTTATTGGTGCCGTGGCCGCTAAGCTATACCCCTCTGTGGCTGCTTTTGCTGTCGTTTGTAGTGTTTGACAGCG
TGCGCAGCCAGCGTCGAATTAACGCCCGTCAGGGTGAAATTAAGCTGCTGATGGATTCTCGCCTGCGCTGGCAGGGAAAA
GAGTGGGAAATGATAGGGACGCCGTGGATGCTCAACACCGGGATGATGCTACGGCTGCGTCGGGTCGAGGACAATCGTCG
CCAGCACCTGTGGCTGGCAGCAGACAGTATGGATCCTGCGGAGTGGCGAGATCTGCGCCGGTTGATCGTGCAACAGCCGA
CGCAGGAGTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11537.99 Da        Isoelectric Point: 9.4646

>AT161033 WP_000063158.1 NZ_CP055019:c4189537-4189235 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDSDVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT161033 NZ_CP071011:c3734876-3734610 [Enterobacter hormaechei]
ATGGATATTAACAACAAGGCCCGTATTCACTGGGCGTGCCGTCGCGGCATGCGTGAACTTGATATCTCCATCATGCCTTT
CTTCGAGTATGAGTACGACAGCTTAAGCGATGACGATAAGCGCCTGTTTGTCCGTCTGCTTGAGTCCGACGATCCGGATT
TATTCAACTGGCTGATGAATCACGGCAAGCCCGCCGACACCGAGTTGCAACGGATGGTGCAATTAATTCAAACACGGAAT
CAGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2U2VE01

Antitoxin

Source ID Structure
AlphaFold DB A0A2U2VE39

References