Detailed information of TA system TA161032

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 3693910..3694747
• Replicon: chromosome
• Accession: NZ_CP055019
• Organism: Escherichia coli strain SWHEFF_69

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: A0A1M0D5U3
• Locus tag: HUZ77_RS17670
• Protein ID: WP_000227782.1
• Coordinates: 3694205..3694747 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: B1LJI1
• Locus tag: HUZ77_RS17665
• Protein ID: WP_001353405.1
• Coordinates: 3693910..3694221 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ77_RS17640 (3688930)	3688930..3689877	+	948	WP_001239437.1	cytochrome o ubiquinol oxidase subunit II	-
HUZ77_RS17645 (3689899)	3689899..3691890	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
HUZ77_RS17650 (3691880)	3691880..3692494	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
HUZ77_RS17655 (3692494)	3692494..3692823	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
HUZ77_RS17660 (3692835)	3692835..3693725	+	891	WP_000971327.1	heme o synthase	-
HUZ77_RS17665 (3693910)	3693910..3694221	+	312	WP_001353405.1	DUF1778 domain-containing protein	Antitoxin
HUZ77_RS17670 (3694205)	3694205..3694747	+	543	WP_000227782.1	GNAT family N-acetyltransferase	Toxin
HUZ77_RS17675 (3694803)	3694803..3695647	-	845	Protein_3471	tetratricopeptide repeat protein	-
HUZ77_RS17680 (3696054)	3696054..3697418	+	1365	WP_001000978.1	MFS transporter	-
HUZ77_RS17685 (3697546)	3697546..3698037	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
HUZ77_RS17690 (3698205)	3698205..3699116	+	912	WP_000705841.1	2-dehydropantoate 2-reductase	-
HUZ77_RS17695 (3699079)	3699079..3699669	+	591	WP_001276320.1	protein deglycase YajL	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (19-96)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19957.31 Da
• Isoelectric Point: 9.1763

>T161032 WP_000227782.1 NZ_CP055019:3694205-3694747 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDIIYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCNFFEHVKIIHRALPIKGVYLDADPAAINFYTRLGFVQLSA
RPNVFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T161032 NZ_CP071011:3454408-3454788 [Enterobacter hormaechei]
ATGCAAACTTTACCGTTACCCGAACCTCGGGCGGCTGAGCCATGTCCGTCGCCCGTGCAGGTCTGGCAACTCCTGCTCAC
GCATCTGCTGGATAAGCATTATGGTCTCACACTCAATGACACGCCGTTTGGCGACGACGGCGTAATTCAGGCGCACATCA
ACGCCGGTATCTCTCTGTGCGACGCCGTCAACTTCATTGTGGAAAGGTATGAACTGTTGCGCACTGACCACAGCAGCTCC
AGCCTCACCGAGCACTCTTCACTGATTGGTGCCATCGACATCCTGCGTGCCCACCGTGCAACCGGCCTGATAGTGGGCAG
CGGCTACAGCACCATCACCCGCATCACAACGGGCCAGTATCGGGAGGACAAAACACAGTGA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11370.09 Da
• Isoelectric Point: 10.4825

>AT161032 WP_001353405.1 NZ_CP055019:3693910-3694221 [Escherichia coli]
MPGKTVALPDIDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT161032 NZ_CP071011:3454049-3454375 [Enterobacter hormaechei]
ATGCATTCTGAACAATGGGGACTGACGTGCGGTAAAACGCCGTGCTTCAGTGCCAAGCTGGTGCAGGAAGGCAACCGGCT
GCACTTTCTAGCTGACCGAGCAGAGTTTAGTGGCGACTTGAGCGACGATGAGGCACTGCGCCTCGACCAGGCTTTTCCGG
TGATATTGAAGCAGCTGGAACAAATGCTTTCAGCCGGTGAGCTGAATCCCCGCCATCAGCATCGCGTCACTCTGCATCTT
AACGGGCTAACCTGCGAAGCCGACACGCTCGGTTCGCACGGCTACCTTTACATCGCGATTTATCCCTCAGCGGTAGCTAC
CGGCTGA

Structures

Toxin

• AlphaFold DB: A0A1M0D5U3

Antitoxin

• AlphaFold DB: A0A829G7G3