Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3659820..3660438 Replicon chromosome
Accession NZ_CP055019
Organism Escherichia coli strain SWHEFF_69

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag HUZ77_RS17500 Protein ID WP_001291435.1
Coordinates 3660220..3660438 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag HUZ77_RS17495 Protein ID WP_000344800.1
Coordinates 3659820..3660194 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ77_RS17485 (3654909) 3654909..3656102 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
HUZ77_RS17490 (3656125) 3656125..3659274 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
HUZ77_RS17495 (3659820) 3659820..3660194 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
HUZ77_RS17500 (3660220) 3660220..3660438 + 219 WP_001291435.1 HHA domain-containing protein Toxin
HUZ77_RS17505 (3660610) 3660610..3661161 + 552 WP_000102574.1 maltose O-acetyltransferase -
HUZ77_RS17510 (3661277) 3661277..3661747 + 471 WP_000136192.1 YlaC family protein -
HUZ77_RS17515 (3661911) 3661911..3663461 + 1551 WP_001361689.1 cyclic-guanylate-specific phosphodiesterase PdeB -
HUZ77_RS17520 (3663503) 3663503..3663856 - 354 WP_000878140.1 DUF1428 domain-containing protein -
HUZ77_RS17530 (3664235) 3664235..3664546 + 312 WP_000409911.1 MGMT family protein -
HUZ77_RS17535 (3664577) 3664577..3665149 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T161031 WP_001291435.1 NZ_CP055019:3660220-3660438 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T161031 NZ_CP071011:c2708351-2708248 [Enterobacter hormaechei]
GGCAAGGCGATTGAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT161031 WP_000344800.1 NZ_CP055019:3659820-3660194 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT161031 NZ_CP071011:2708243-2708444 [Enterobacter hormaechei]
CAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTT
GGCATTAATGCAGGCTCAATCGCCTTGCCCTTTAAGAATAGATGACGACGTCAGGTTTTCCAGTCCACAGTAAAAGTGGT
CTGAAAAAAAGCGTCAGAACATCACTAAATGTGAAAAACCGC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References