Detailed information of TA system TA161029

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 3234898..3235603
• Replicon: chromosome
• Accession: NZ_CP055019
• Organism: Escherichia coli strain SWHEFF_69

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A1M2J6W7
• Locus tag: HUZ77_RS15390
• Protein ID: WP_000539524.1
• Coordinates: 3234898..3235284 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: HUZ77_RS15395
• Protein ID: WP_001280945.1
• Coordinates: 3235274..3235603 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ77_RS15370 (3230902)	3230902..3231528	+	627	WP_001295292.1	glutathione S-transferase GstB	-
HUZ77_RS15375 (3231525)	3231525..3232640	-	1116	WP_000555056.1	aldose sugar dehydrogenase YliI	-
HUZ77_RS15380 (3232751)	3232751..3233134	-	384	WP_000497137.1	biofilm formation regulator BssR	-
HUZ77_RS15385 (3233347)	3233347..3234672	+	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
HUZ77_RS15390 (3234898)	3234898..3235284	+	387	WP_000539524.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HUZ77_RS15395 (3235274)	3235274..3235603	+	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
HUZ77_RS15400 (3235673)	3235673..3236990	-	1318	Protein_3032	diguanylate cyclase	-
HUZ77_RS15405 (3236998)	3236998..3239346	-	2349	WP_000950331.1	EAL domain-containing protein	-
HUZ77_RS15410 (3239523)	3239523..3240434	-	912	WP_001236044.1	glutathione ABC transporter permease GsiD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14321.46 Da
• Isoelectric Point: 9.9521

>T161029 WP_000539524.1 NZ_CP055019:3234898-3235284 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSRSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T161029 NZ_CP071011:c1080527-1080309 [Enterobacter hormaechei]
ATGTCTGATAAACCATTAACTAAAGTCGATTATTTGATGCGCCTGCGACGCTGTCAGTCAATTGACACGCTCGAACGAGT
TATTGAGAAAAATAAATACGAGCTGTCTGATAATGAACTGGCGGTATTTTATTCAGCTGCCGACCATCGTCTTGCTGAAC
TGACCATGAATAAACTCTATGACAAGATCCCCTCTTCGGTATGGAAATTTGTTCGTTAG

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT161029 WP_001280945.1 NZ_CP055019:3235274-3235603 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT161029 NZ_CP071011:c1080930-1080556 [Enterobacter hormaechei]
TTGGACGAGTACTCGCCAAAAAGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAATCTTGAAGAAAGTAACCATGGCTGGGTGAACGATCCAACGTCTGCTATCAATTTACAGCTTAATGAGCTGATCGAGC
ATATCGCAACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAGTTGATAGAGCAAATTGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTACGGCATTAACGCACAGGATTTGCAGAAATGGCGTAAATCAGGAAACCGTCT
ATTTCGCTGCTTCACGAATGTCAGCAGAGCTAATCCGGTTAGTCTTTCCTGTTAA

Structures

Toxin

• AlphaFold DB: A0A1M2J6W7