161017

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pmenTA/darT(toxin)
Location 1979136..1980856 Replicon chromosome
Accession NZ_CP055019
Organism Escherichia coli strain SWHEFF_69

Toxin (Protein)


Gene name pmenT Uniprot ID A0A7U9J2A7
Locus tag HUZ77_RS09225 Protein ID WP_000312833.1
Coordinates 1980200..1980856 (-) Length 219 a.a.

Antitoxin (Protein)


Gene name pmenA Uniprot ID B7UP19
Locus tag HUZ77_RS09220 Protein ID WP_000634203.1
Coordinates 1979136..1980203 (-) Length 356 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ77_RS09205 (1974347) 1974347..1974961 - 615 Protein_1806 IS3-like element IS600 family transposase -
HUZ77_RS09210 (1975306) 1975306..1976022 - 717 WP_001122107.1 SprT family zinc-dependent metalloprotease -
HUZ77_RS09215 (1976022) 1976022..1979117 - 3096 WP_000108736.1 HsdR family type I site-specific deoxyribonuclease -
HUZ77_RS09220 (1979136) 1979136..1980203 - 1068 WP_000634203.1 toxin-antitoxin system antitoxin DNA ADP-ribosyl glycohydrolase DarG Antitoxin
HUZ77_RS09225 (1980200) 1980200..1980856 - 657 WP_000312833.1 toxin-antitoxin system toxin DNA ADP-ribosyl transferase DarT Toxin
HUZ77_RS09230 (1980869) 1980869..1982224 - 1356 WP_223349791.1 restriction endonuclease subunit S -
HUZ77_RS09235 (1982224) 1982224..1984662 - 2439 WP_032180025.1 type I restriction-modification system subunit M -
HUZ77_RS09240 (1984702) 1984702..1984941 - 240 WP_024224214.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island blaCMY-2 csgG / csgF / csgE / csgD / csgB / csgA / csgC 1953033..2010625 57592


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

ssDNA thymidine ADP-ribosyltransferase, DarT

(17-217)

Antitoxin

Macro domain

(18-129)


Sequences

Toxin        


Download         Length: 219 a.a.        Molecular weight: 25233.56 Da        Isoelectric Point: 6.2145

>T161017 WP_000312833.1 NZ_CP055019:c1980856-1980200 [Escherichia coli]
MAYDYSASLNPQKALIWRIVHRDNIPWILDNGLHCGNSLVQAENWINIGNPELIGKRAGHPVPVGTGGTLHDYVPFYFTP
FSPMLMNIHSGRGGIKQRPNEEIVILVSNLRNVAAHDVPFVFTDSHAYYNWTNYYTSLDSLDQIDWPILQARDFRRDPDD
PAKFERYQAEALIWQHCPISLLDGIICYSEEVRLQLEQWLFQRNLTMSVHTRAGWYFS

Download         Length: 657 bp

>T161017 NZ_CP071007:4479545-4480414 [Pseudomonas sp. SORT22]
ATGTTGCACGCCACCGACCACTCGCTCGCCGGCCTTGCGGCCCAGTTGCAGCAGCGCTTCCCCCAGCGCCTGGTGCAACT
GGCAAGCGGCGCCCAGGCGATCCGCGAAGCCGGGCACGGGCCGGCCATCGTGCTGTTGCATGGCATTGGCTCGGGCGCCG
CGTCCTGGCTGCAGGTGGCGCTGCAACTGAGCCAGCACGCCCGGGTGATTGCCTGGGATGCACCGGGCTACGGCGACTCC
AGCCCACTGGCCAACCCTGCGCCGCAGGCGGCGGACTATGCCCGGCGCCTGCGGCATTTGCTCGATGCGCTGCAGATTGA
CGACTGCCTGCTGGTCGGCCATTCCCTCGGTGCCCTCAGCGCCGCGGCCCTGGCCCAGCAGCAGCCGCAACGGGTGCGGC
GCCTGGTGCTGATCAGCCCGGCTCGCGGCTATGGCGACCCGGCCCGTGCCGAGCAGGCGCAACAGGTGCGCAACCAGCGC
CTGCACAACCTTGAGCAACTGGGCATCGCCCAGATGGCCAACCAGCGCAGCGCACACATGCTCTCGCCAGCGGCCAGCAG
CGAAGCCCGCGCCTGGGTGCAATGGAACATGGCGCGGCTGCTGCCCCAGGGCTATCGCCAGGCCATCGAGTTGCTGTGTG
GCGATGACCTGCTGCGCTACGCCGCCCTGGCCGTGCCGTGCGAAGTGCATTGCGGCGCCGCTGACAGCATCACCCTCCCC
GAGGACTGCCAGGCCCTGGCCAAGGCCCTGGGGGCAGCGTTCGCGTTGATTGCCGAGGCCGGCCACGCCAGCCCGATCGA
ACAACCCGAGGCCGTGGCAAGCCTGCTTGCCCGGGCCCTCGAAGAATCCCTGACAGGTACTGCGCTATGA

Antitoxin


Download         Length: 356 a.a.        Molecular weight: 40672.70 Da        Isoelectric Point: 5.6927

>AT161017 WP_000634203.1 NZ_CP055019:c1980203-1979136 [Escherichia coli]
MITYTQGNLLDAPVEALVNTVNTVGVMGKGIALMFKERFPENMKVYALACKQKQVITGKMFITETGELMGPRWIVNFPTK
QHWRADSRMEWIEDGLQDLRRFLIEENVQSIAIPPLGAGNGGLNWPDVRAQIESALGDLQDVDILIYQPTEKYQNVAKST
GVKKLTPARAAIAELVRRYWVLGMECSLLEIQKLAWLLQRAIEQHQQDDILKLRFEAHYYGPYAPNLNHLLNALDGTYLK
AEKRIPDSQPLDVIWFNDQKKEHVNAYLNNEAREWLPALEQVSQLIDGFESPFGLELLATVDWLLSRGECQPTLDSVKEG
LHQWPAGERWASRKLRLFDNNNLQFAINRVMEFHC

Download         Length: 1068 bp

>AT161017 NZ_CP071007:4480411-4481238 [Pseudomonas sp. SORT22]
ATGAATGATTCGGATCTGTTGAAAGACAACCAGGACAAGTACATCGTCCCCGGCCTTGAGCGCGGCCTGTTGCTGCTCTG
TGAATTCAGCCGGCAAAACCGCACCCTCACCGCGCCGGAACTGGCGCGGCGCCTGGAGCTGCCGCGCTCGACCATCTTCC
GCCTGTTGACCACCCTGGAAACCATGGGCTTCGTCACCCGCAGCGGCAACGAGTACCGCCTGGGCATGTCAGTACTGCGC
CTGGGCTTCGAGTACCTGGCATCGCTGGAGCTGACCGAGCTCGGTCAGCCGCTGCTGGCGCGGCTGTGCGATCACCTTAA
TTACCCGAGCAACCTGGTGGTGCGTGACGGTCGCTCGATCGTCTACGTGGCCAAGGTTTCGCCGCCGTCGGTGTTCTCCA
GCGCGGTCAACGTCGGCACCCGCCTGCCGGCCCACGCCACGGTGCTGGGGCGCATCCTCCTTGAAGACCTGAGCCTGGCC
GAGCTGCGTGAGCTGTACCCGGAGGAGCACCTGGAACAGCACTCGCCGTGCACACCGAAAACCGTACTGGAGCTGTTCGA
CCTGGTGCAGAGCGACCGTCAGCGCGGCTATGTCAGCGGCGAAGGTTTTTTCGAATCATCGATCTCGACCATCGCTGCGC
CGGTGCGTGATCACAGTGGCCGGGTGGTTGCCGCCATGGGCGTGACCATCCCTACCGTGCAGGTTGCGCACATCAATTTC
GACGGCCTGCTTGGCCATGTGCGGGGCAGCGCCGATGAGCTGTCGCGCCTGCTCAACTACACGCCAAACGCTGGCTCCGG
CCGCGTCACCGCGCTGATGAGGGACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9J2A7

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9J2B9

References