Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 962184..962838 Replicon chromosome
Accession NZ_CP055019
Organism Escherichia coli strain SWHEFF_69

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag HUZ77_RS04690 Protein ID WP_000244781.1
Coordinates 962431..962838 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag HUZ77_RS04685 Protein ID WP_000354046.1
Coordinates 962184..962450 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ77_RS04665 (958272) 958272..959705 - 1434 WP_001521235.1 6-phospho-beta-glucosidase BglA -
HUZ77_RS04670 (959750) 959750..960061 + 312 WP_001182949.1 N(4)-acetylcytidine aminohydrolase -
HUZ77_RS04675 (960225) 960225..960884 + 660 WP_000250269.1 hemolysin III family protein -
HUZ77_RS04680 (960961) 960961..961941 - 981 WP_000886050.1 tRNA-modifying protein YgfZ -
HUZ77_RS04685 (962184) 962184..962450 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
HUZ77_RS04690 (962431) 962431..962838 + 408 WP_000244781.1 protein YgfX Toxin
HUZ77_RS04695 (962878) 962878..963399 - 522 WP_001521233.1 flavodoxin FldB -
HUZ77_RS04700 (963511) 963511..964407 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
HUZ77_RS04705 (964432) 964432..965142 + 711 WP_001521231.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
HUZ77_RS04710 (965148) 965148..966881 + 1734 WP_063117505.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T161015 WP_000244781.1 NZ_CP055019:962431-962838 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T161015 NZ_CP071000:c1735132-1734869 [Limosilactobacillus fermentum]
ATGAGTTACGCGATCAAACTCAAGCGGAGCGCTAATAAAGATCTCAAGAAAATTAAGGGGTCATATTTAGAAAAGAGCTT
CTTGCAAATAATTGAGCAGCTCCGTAAAGACCCCTTGGCACCCAACCAAGGCTTTGAAAAATTAGTACTGCCAATTAAGG
GCTTTTACTCCCGGCGGATTAACATTCAGCACCGACTCGTCTATAAGGTTGATCAAGACACGCAAACTGTGATCATCTAT
TCTGCGTGGTCACATGACGAATAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT161015 WP_000354046.1 NZ_CP055019:962184-962450 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT161015 NZ_CP071000:c1735383-1735129 [Limosilactobacillus fermentum]
ATGGACCAAATTGTAACCCCAACGCAAGCACGGAAAGATTTATTCAAAATAATCAAAAGGATCAATGAAAATAATGAGCC
GGTTGTGATTAAGCCGACCAGGGCGGATGAAAAGAGCGTTGTCGTCATTGGTGAAGATGACTGGAAAGCAATCCAAGAAA
CGTTATTTTTGGTTAATCACGGTGTTGACAAGCAAATTAAAGAACGGGAAAACGAGCCGCTCGACGACTTTGATGATGTT
TGGGGTCAACTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References