Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 212239..212851 Replicon chromosome
Accession NZ_CP055019
Organism Escherichia coli strain SWHEFF_69

Toxin (Protein)


Gene name hicA Uniprot ID U9YXE2
Locus tag HUZ77_RS00995 Protein ID WP_000833473.1
Coordinates 212239..212424 (+) Length 62 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A1M2IVK0
Locus tag HUZ77_RS01000 Protein ID WP_000499742.1
Coordinates 212441..212851 (+) Length 137 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ77_RS00980 (207705) 207705..209000 + 1296 WP_000985745.1 Fic family protein -
HUZ77_RS00985 (209108) 209108..210646 + 1539 WP_000183978.1 aldehyde dehydrogenase AldB -
HUZ77_RS00990 (210687) 210687..211766 - 1080 WP_000061475.1 type I restriction enzyme HsdR N-terminal domain-containing protein -
HUZ77_RS00995 (212239) 212239..212424 + 186 WP_000833473.1 type II toxin-antitoxin system HicA family toxin Toxin
HUZ77_RS01000 (212441) 212441..212851 + 411 WP_000499742.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
HUZ77_RS01005 (212963) 212963..214942 - 1980 WP_001026863.1 glycoside hydrolase family 127 protein -
HUZ77_RS01010 (214953) 214953..216353 - 1401 WP_000204814.1 MFS transporter -
HUZ77_RS01015 (216579) 216579..217394 + 816 WP_000891820.1 AraC family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(4-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-129)


Sequences

Toxin        


Download         Length: 62 a.a.        Molecular weight: 6800.89 Da        Isoelectric Point: 11.7053

>T161010 WP_000833473.1 NZ_CP055019:212239-212424 [Escherichia coli]
VKSADVIAILKQHGWEHIRTRGSHHQFRHPVHPGLVTVPHPKKDIKPGTLAQIGRQAGLTF

Download         Length: 186 bp

>T161010 NZ_CP070997:c143682-143389 [Acinetobacter indicus]
ATGACTTATAAGCTTTTACGTCACAAGGACTTTACAGCAGAATGGGAAAAACTCCCTGTTGCTATACGAGATCAATTTAA
AAAGAAACTAGCCAAAGTCATAGAGCAGCCACATATTCCAAAGAATATGCTTAGAGGCGATCTTGCAGGTTGCTATAAAA
TCAAATTATTAAAAGCTGGTGTCAGACTTGTCTATCAAGTTAAAGATGATCAGGTAGTTATCTTGCTCATCACAGTCGGG
AAACGAGCTGATAGCATAGTTTATGACGAAGCTAAAAAGCGAATTAAAGACTAA

Antitoxin


Download         Length: 137 a.a.        Molecular weight: 15242.13 Da        Isoelectric Point: 4.4482

>AT161010 WP_000499742.1 NZ_CP055019:212441-212851 [Escherichia coli]
MFYPAYIHSDPDGSASGFFPDVPGCYFAGDTLDNAFQDARSALVAHFETLCEMDEELPLPGSVETHLVQRAQDFIGGQWL
LVDINMNQFEGRAERINITMPKRLLNKIDTYVRNNPDYANRSAFLAEAARRVLPGV

Download         Length: 411 bp

>AT161010 NZ_CP070997:c143917-143672 [Acinetobacter indicus]
ATGGCTACTGTTAACTTCAGGGTCGATGAAGCCCTTAAAGAAAAATCTTATTCAATCCTTAAAGAGCAAGGTATTGCACC
TACGGATTTCTTCACTAGTATTCTTGAGTATGTTGCTACGACTGGGAAACTGCCTGTCAAAAAAGCTTTGCTTTCGGAAG
AAGATGAAGAATTATTAGCTCTTGTCCGTAAGCGCATTAATGATCCTAAAGAGATGTTTGAGGAAGTCACATTAGATGAC
TTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9YXE2

Antitoxin

Source ID Structure
AlphaFold DB A0A1M2IVK0

References