Detailed information of TA system TA161009

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 70655..71453
• Replicon: chromosome
• Accession: NZ_CP055019
• Organism: Escherichia coli strain SWHEFF_69

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: HUZ77_RS00305
• Protein ID: WP_061092894.1
• Coordinates: 70655..71032 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: HUZ77_RS00310
• Protein ID: WP_061092893.1
• Coordinates: 71079..71453 (-)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ77_RS00275 (66018)	66018..66941	-	924	WP_000535959.1	carboxylate/amino acid/amine transporter	-
HUZ77_RS00280 (67052)	67052..68236	-	1185	WP_001172864.1	sugar efflux transporter	-
HUZ77_RS00285 (68635)	68635..68796	-	162	Protein_56	RhuM family protein	-
HUZ77_RS00290 (69034)	69034..69876	-	843	WP_061092922.1	DUF4942 domain-containing protein	-
HUZ77_RS00295 (69961)	69961..70158	-	198	WP_012908799.1	DUF957 domain-containing protein	-
HUZ77_RS00300 (70170)	70170..70658	-	489	WP_061092895.1	DUF5983 family protein	-
HUZ77_RS00305 (70655)	70655..71032	-	378	WP_061092894.1	TA system toxin CbtA family protein	Toxin
HUZ77_RS00310 (71079)	71079..71453	-	375	WP_061092893.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HUZ77_RS00315 (71616)	71616..71837	-	222	WP_061092892.1	DUF987 domain-containing protein	-
HUZ77_RS00320 (71900)	71900..72376	-	477	WP_001186188.1	RadC family protein	-
HUZ77_RS00325 (72391)	72391..72870	-	480	WP_000706978.1	antirestriction protein	-
HUZ77_RS00330 (73136)	73136..73954	-	819	WP_001175165.1	DUF932 domain-containing protein	-
HUZ77_RS00335 (74044)	74044..74277	-	234	WP_001278293.1	DUF905 family protein	-
HUZ77_RS00340 (74283)	74283..74591	-	309	Protein_67	hypothetical protein	-
HUZ77_RS00345 (74659)	74659..75836	+	1178	WP_152895938.1	IS3 family transposase	-
HUZ77_RS00350 (75844)	75844..76221	-	378	Protein_69	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	59309..86277	26968

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14196.15 Da
• Isoelectric Point: 7.8045

>T161009 WP_061092894.1 NZ_CP055019:c71032-70655 [Escherichia coli]
MKTLSDTHVREVSRCPSPVTIWQTLLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALIRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITQGKHPEAKQ

Nucleotide

• Length: 378 bp

>T161009 NZ_CP070996:c2069935-2069612 [Bifidobacterium longum subsp. suillum]
ATGAAACGAGGTGAGATTTGGACGTTGCAGGCCGATGGCTACGCCAGCAAGCCACGGCCGGTTGTCATCGTGCAGAGCGA
TGCCGTCGATCGGTTCGATTCCGTCATCACTTGTCTGCTGACCTCATACGATTCCTCCGACATCGACACGCGGGTCCGAT
TGGAACCAAGTCCGGAGAACGGCCTGAACAAAGTGAGTTACGTGATGACGGACAAGATCGTCACCGTCGACCGCAAACTG
CTGGGATACCAAGTCGGCGTGGTTGATGATGCAGCCATGGCGAATATCGGCCATCAGCTCATGCGGGTTCTAGGTTTGCT
GTGA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 13752.53 Da
• Isoelectric Point: 6.5924

>AT161009 WP_061092893.1 NZ_CP055019:c71453-71079 [Escherichia coli]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNHLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLTVYPTPAPATTS

Nucleotide

• Length: 375 bp

>AT161009 NZ_CP070996:c2070167-2069922 [Bifidobacterium longum subsp. suillum]
ATGAGCATGCAGATTGCCACGCGAGTTGATGACGAGCAGGCTGCGTTGTTTAAGGAGACGACCCGACAGCTGGGTACCAC
CCCGGCCGATGCCCTGCGTATGTTCATCAGCGCGTTCAATGATTACCGTGGGTTCCCCTATGAGGTGCGTTTGCCACGAA
ATGACGTGGAGCCGTTTGCGTCCGAGCGGGACGCTACTGAGTATGCCAGTCGTCTCGCCTTGAGGATGTCCGATGAAACG
AGGTGA