Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 30387..31030 Replicon plasmid unnamed1
Accession NZ_CP055016
Organism Shigella flexneri strain SWHEFF_71

Toxin (Protein)


Gene name vagD Uniprot ID V0UN72
Locus tag HUZ78_RS24565 Protein ID WP_001034044.1
Coordinates 30614..31030 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID B1P7N7
Locus tag HUZ78_RS24560 Protein ID WP_001261286.1
Coordinates 30387..30617 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ78_RS24525 (25560) 25560..25727 + 168 Protein_27 colicin-B -
HUZ78_RS24530 (25745) 25745..26272 - 528 WP_000203272.1 colicin B immunity protein -
HUZ78_RS24535 (26516) 26516..27331 + 816 WP_001312845.1 lipid II-degrading bacteriocin colicin M -
HUZ78_RS24540 (27381) 27381..27734 - 354 WP_000864812.1 colicin M immunity protein -
HUZ78_RS24545 (27912) 27912..28703 - 792 WP_000016493.1 site-specific integrase -
HUZ78_RS24550 (28700) 28700..29389 - 690 WP_089583722.1 hypothetical protein -
HUZ78_RS24555 (29433) 29433..29783 - 351 WP_000493379.1 hypothetical protein -
HUZ78_RS24560 (30387) 30387..30617 + 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
HUZ78_RS24565 (30614) 30614..31030 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin Toxin
HUZ78_RS24570 (31105) 31105..32670 + 1566 WP_001128474.1 AAA family ATPase -
HUZ78_RS24575 (32655) 32655..33677 + 1023 WP_000361402.1 helicase UvrD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(A) / aadA2 / dfrA12 / aac(3)-IId / blaTEM-1B / aph(6)-Id / aph(3'')-Ib / sul2 / sitABCD exeG / exeE / stcE 1..183928 183928


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15232.66 Da        Isoelectric Point: 6.8536

>T161004 WP_001034044.1 NZ_CP055016:30614-31030 [Shigella flexneri]
VNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR

Download         Length: 417 bp

>T161004 NZ_CP070991:2669227-2669436 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATAGATGGTCAAGTAACTGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8747.86 Da        Isoelectric Point: 4.3775

>AT161004 WP_001261286.1 NZ_CP055016:30387-30617 [Shigella flexneri]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMTEREDVVSDEGRVNL

Download         Length: 231 bp

>AT161004 NZ_CP070991:2668922-2669215 [Staphylococcus aureus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGACAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CHW1

Antitoxin

Source ID Structure
AlphaFold DB A0A829CKZ6

References