Detailed information of TA system TA161003

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RelE-RHH
• Location: 13460..14007
• Replicon: plasmid unnamed1
• Accession: NZ_CP055016
• Organism: Shigella flexneri strain SWHEFF_71

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: HUZ78_RS24460
• Protein ID: WP_097309154.1
• Coordinates: 13460..13738 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: E1QMW3
• Locus tag: HUZ78_RS24465
• Protein ID: WP_000079941.1
• Coordinates: 13738..14007 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ78_RS24435 (9703)	9703..10737	-	1035	WP_001190234.1	3-deoxy-7-phosphoheptulonate synthase	-
HUZ78_RS24440 (10918)	10918..11218	+	301	Protein_10	hypothetical protein	-
HUZ78_RS24445 (11296)	11296..11643	-	348	WP_000142437.1	DUF6404 family protein	-
HUZ78_RS24450 (11967)	11967..12449	-	483	WP_001311056.1	hypothetical protein	-
HUZ78_RS24455 (12566)	12566..13414	-	849	WP_001058005.1	3'-5' exonuclease	-
HUZ78_RS24460 (13460)	13460..13738	-	279	WP_097309154.1	type II toxin-antitoxin system toxin YacB	Toxin
HUZ78_RS24465 (13738)	13738..14007	-	270	WP_000079941.1	type II toxin-antitoxin system antitoxin YacA	Antitoxin
HUZ78_RS24470 (14529)	14529..15765	+	1237	Protein_16	IS3 family transposase	-
HUZ78_RS24475 (15962)	15962..16441	+	480	Protein_17	colicin V secretion protein CvaA	-
HUZ78_RS24485 (17746)	17746..18549	+	804	Protein_19	HlyD family secretion protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A) / aadA2 / dfrA12 / aac(3)-IId / blaTEM-1B / aph(6)-Id / aph(3'')-Ib / sul2 / sitABCD	exeG / exeE / stcE	1..183928	183928

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10799.42 Da
• Isoelectric Point: 8.4615

>T161003 WP_097309154.1 NZ_CP055016:c13738-13460 [Shigella flexneri]
MEIFWTMLASQDRKRIREYIAEQNLIAAIELDERIGYSASNLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKVY
ILRVLHTVQKWP

Nucleotide

• Length: 279 bp

>T161003 NZ_CP070991:2362942-2363208 [Staphylococcus aureus]
ATGGCTAGGTTAAATATTACGTTTTCGCCTCAAGCCTTTGAAGATTATAAGTATTTTCAGCAGAACGATAAAAAAATGGT
GAAGAAGATTAATGAGTTACTTAAAAGTATTGACAGAAATGGCGCATTGAAAGGTATAGGTAAGCCTGAAAAGTTAAAAT
CGAATCTGACTGGGTATTATAGTAGACGTATCAATCACGAACATAGATTGGTTTATACAGTAGATGACAATCATATAAAA
ATAGCATCATGTAAATACCATTATTAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10252.58 Da
• Isoelectric Point: 7.2057

>AT161003 WP_000079941.1 NZ_CP055016:c14007-13738 [Shigella flexneri]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLERGDVLPHDMVESSAAAWRD
EMSRKVAGK

Nucleotide

• Length: 270 bp

>AT161003 NZ_CP070991:2362691-2362942 [Staphylococcus aureus]
ATGATTATTAAAAATTATTCATACGCTCGACAGAATTTAAAGGCACTTATGACAAAAGTAAATGATGATAGTGATATGGT
AACTGTAACATCTACTGATGATAAAAACGTAGTAATCATGTCAGAATCAGATTATAACTCCATGATGGAAACACTTTACC
TCCAACAGAATCCAAATAATGCTGAACACTTAGCTCAATCAATTGCAGATCTAGAACGTGGGAAAACTATAACGAAAGAT
ATAGATGTATAA

Structures

Antitoxin

• AlphaFold DB: A0A629TXC5