Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4108161..4108779 Replicon chromosome
Accession NZ_CP055015
Organism Shigella flexneri strain SWHEFF_71

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag HUZ78_RS20165 Protein ID WP_001291435.1
Coordinates 4108161..4108379 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag HUZ78_RS20170 Protein ID WP_000344800.1
Coordinates 4108405..4108779 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ78_RS20130 (4103450) 4103450..4104022 + 573 WP_000779831.1 YbaY family lipoprotein -
HUZ78_RS20135 (4104053) 4104053..4104364 - 312 WP_000409911.1 MGMT family protein -
HUZ78_RS20145 (4104743) 4104743..4105096 + 354 WP_000878140.1 DUF1428 domain-containing protein -
HUZ78_RS20150 (4105138) 4105138..4106688 - 1551 WP_205850266.1 EAL domain-containing protein -
HUZ78_RS20155 (4106852) 4106852..4107322 - 471 WP_000136192.1 YlaC family protein -
HUZ78_RS20160 (4107438) 4107438..4107989 - 552 WP_000102568.1 maltose O-acetyltransferase -
HUZ78_RS20165 (4108161) 4108161..4108379 - 219 WP_001291435.1 HHA domain-containing protein Toxin
HUZ78_RS20170 (4108405) 4108405..4108779 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
HUZ78_RS20175 (4109325) 4109325..4112474 - 3150 WP_001132469.1 efflux RND transporter permease AcrB -
HUZ78_RS20180 (4112497) 4112497..4113690 - 1194 WP_096941974.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T161002 WP_001291435.1 NZ_CP055015:c4108379-4108161 [Shigella flexneri]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T161002 NZ_CP070991:2337816-2337923 [Staphylococcus aureus]
ATGTTCAATTTATTAATTGACATCATGACTTCAGCTTTAAGCGGCTGTCTTGTTGCGTTTTTTGCACATTGGTTACGAAC
GCGCAACAATAAAAAAGGTGACAAATAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT161002 WP_000344800.1 NZ_CP055015:c4108779-4108405 [Shigella flexneri]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT161002 NZ_CP070991:c2338000-2337940 [Staphylococcus aureus]
TACATAATAAATTGAACATCTAAATACACCAAATCCCCTCACTACTGCCATAGTGAGGGGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References