161001

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 4073861..4074698 Replicon chromosome
Accession NZ_CP055015
Organism Shigella flexneri strain SWHEFF_71

Toxin (Protein)


Gene name itaT Uniprot ID A0A0F5GET7
Locus tag HUZ78_RS19995 Protein ID WP_000227783.1
Coordinates 4073861..4074403 (-) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag HUZ78_RS20000 Protein ID WP_001297137.1
Coordinates 4074387..4074698 (-) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ78_RS19975 (4069400) 4069400..4070311 - 912 WP_000705874.1 2-dehydropantoate 2-reductase -
HUZ78_RS19980 (4070479) 4070479..4070970 + 492 WP_001138904.1 nucleotide binding protein YajQ -
HUZ78_RS19985 (4071098) 4071098..4072462 - 1365 WP_001000978.1 MFS transporter -
HUZ78_RS19990 (4072870) 4072870..4073805 + 936 WP_001297127.1 tetratricopeptide repeat protein -
HUZ78_RS19995 (4073861) 4073861..4074403 - 543 WP_000227783.1 GNAT family N-acetyltransferase Toxin
HUZ78_RS20000 (4074387) 4074387..4074698 - 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
HUZ78_RS20005 (4074883) 4074883..4075773 - 891 WP_000971336.1 heme o synthase -
HUZ78_RS20010 (4075785) 4075785..4076114 - 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
HUZ78_RS20015 (4076114) 4076114..4076728 - 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
HUZ78_RS20020 (4076718) 4076718..4078709 - 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
HUZ78_RS20025 (4078731) 4078731..4079678 - 948 WP_096941979.1 cytochrome o ubiquinol oxidase subunit II -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(14-97)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19776.96 Da        Isoelectric Point: 8.0330

>T161001 WP_000227783.1 NZ_CP055015:c4074403-4073861 [Shigella flexneri]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKQGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

>T161001 NZ_CP070991:2306874-2307140 [Staphylococcus aureus]
ATGAGCAATTACACGGTTAAGATTAAAAATTCAGCGAAATCAGATTTAAAGAAAATAAAACATTCTTATTTAAAGAAGTC
ATTTTTAGAAATTGTTGAGACTTTAAAAAATGATCCGTATAAAATAACACAATCCTTTGAAAAATTAGAACCTAAATATT
TAGAGCGATATTCAAGAAGAATTAACCATCAGCACAGGGTCGTCTATACCGTAGATGATCGAAATAAAGAAGTATTGATA
CTATCGGCATGGTCACATTATGATTAA

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT161001 WP_001297137.1 NZ_CP055015:c4074698-4074387 [Shigella flexneri]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp

>AT161001 NZ_CP070991:2306617-2306874 [Staphylococcus aureus]
ATGATTATCACTAGCCCTACAGAAGCGAGAAAAGATTTTTATCAATTACTAAAAAATGTTAATAATAATCACGAACCAAT
TTATATTAGTGGCAATAATGCCGAAAATAATGCTGTGATTATAGGTTTAGAAGATTGGAAAAGTATACAAGAGACAATAT
ATCTTGAATCTACTGGTACAATGGACAAAGTAAGAGAAAGAGAAAAAGATAGTAGTGGTACAACAAATATAGATGATATT
GATTGGGATAATCTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0F5GET7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References