Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 3357038..3357535 Replicon chromosome
Accession NZ_CP055015
Organism Shigella flexneri strain SWHEFF_71

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag HUZ78_RS16630 Protein ID WP_001173343.1
Coordinates 3357362..3357535 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID J7RC38
Locus tag HUZ78_RS16625 Protein ID WP_001425728.1
Coordinates 3357038..3357334 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ78_RS16605 (3353769) 3353769..3354488 - 720 WP_000611288.1 two-component system response regulator DcuR -
HUZ78_RS16610 (3354485) 3354485..3356116 - 1632 WP_001216486.1 sensor histidine kinase -
HUZ78_RS16615 (3356297) 3356297..3356527 + 231 WP_000371698.1 4Fe-4S mono-cluster protein YjdI -
HUZ78_RS16620 (3356539) 3356539..3356811 + 273 WP_000405647.1 GNAT family N-acetyltransferase -
HUZ78_RS16625 (3357038) 3357038..3357334 + 297 WP_001425728.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
HUZ78_RS16630 (3357362) 3357362..3357535 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
HUZ78_RS16635 (3357654) 3357654..3359171 - 1518 WP_001295074.1 lysine--tRNA ligase -
HUZ78_RS16640 (3359408) 3359408..3360865 - 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T160998 WP_001173343.1 NZ_CP055015:3357362-3357535 [Shigella flexneri]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T160998 NZ_CP070991:c206729-206634 [Staphylococcus aureus]
GTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAG
ACGCAATACAAAATAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11466.99 Da        Isoelectric Point: 4.2544

>AT160998 WP_001425728.1 NZ_CP055015:3357038-3357334 [Shigella flexneri]
MEGKNKFNTYVVSFNYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT160998 NZ_CP070991:206549-206606 [Staphylococcus aureus]
AACGAAAATAAGTATTTACTTATACACCAATCCCCTCACTATTTGCGGTAGTGAGGGG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB J7RC38

References